Srivastava A K, Vishwakarma S K, Pandey V K, Singh M P
V.B.S. Purvanchal University Department of Biotechnology Jaunpur India.
V.B.S. Purvanchal University Department of Environmental Science Jaunpur India.
Cell Mol Biol (Noisy-le-grand). 2014 Dec 24;60(5):15-21.
In the present investigation the efficiency of three species of Pleurotus and their improved dikaryons (heterokaryons) was assessed for decolorization of direct red and production of lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase enzymes. All the species of Pleurotus i.e. P. flabellatus, P. ostreatus, and P. citrinopileatus decolorized the dye Direct Red well. However, Pfo 6X9 and Poc 9X6 decolorized the dye more effectively than three species of Pleurotus. The improved dikaryons also showed higher ligninolytic activity than the parental species. Poc 9X6 showed higher LiP (76.27U), MnP (623.24U) and laccase activity (594.80U). In the present work different pH, age and concentration of inoculum and effect of surfactant i.e. sodium dodecyl sulphate (SDS) and Tween—80 were analyzed in order to determine the optimum ones to decolorize maximum concentration of dye. 5 ml of 10 days old culture on pH 5.5 and 0.1% Tween—80 supported maximum decolorization of direct red dye.
在本研究中，评估了三种侧耳属物种及其改良双核体（异核体）对直接红的脱色效率以及木质素过氧化物酶（LiP）、锰过氧化物酶（MnP）和漆酶的产生情况。所有侧耳属物种，即扇形侧耳、糙皮侧耳和金顶侧耳，均能很好地使直接红染料脱色。然而，Pfo 6X9和Poc 9X6比三种侧耳属物种更有效地使染料脱色。改良双核体的木质素分解活性也高于亲本物种。Poc 9X6表现出更高的LiP（76.27U）、MnP（623.24U）和漆酶活性（594.80U）。在本研究中，分析了不同的pH值、菌龄和接种物浓度以及表面活性剂即十二烷基硫酸钠（SDS）和吐温-80的影响，以确定使最大浓度染料脱色的最佳条件。在pH 5.5和0.1%吐温-80条件下，5 ml 10日龄培养物能使直接红染料达到最大脱色效果。
