Mahant Shweta, Sharma Amresh Kumar, Gehlot Valentina, Mukhopadhyay Asish Kumar, Chhawchharia Ayushi, Dutta Sangitanjan, Agarwal Anil, Som Anup, Das Kunal, Das Rajashree
Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India.
Centre of Bioinformatics, University of Allahabad, Allahabad, Uttar Pradesh, India.
Indian J Med Microbiol. 2019 Jul-Sep;37(3):337-344. doi: 10.4103/ijmm.IJMM_19_158.
Helicobacter pylori causes various gastro-intestinal diseases. Antibiotic resistance to commonly used antibiotics for the treatment of H. pylori infection is the major cause for treatment failure. The aim of this study is to determine the antimicrobial susceptibility pattern for clarithromycin and levofloxacin and find the evolutionary relationship of the partial sequence of 23S rRNA and gyraseA gene of H. pylori by phylogenetic analysis.
A total of 46 H. pylori strains were tested for clarithromycin and levofloxacin susceptibility pattern and phylogenetic tree were reconstructed by PhyML software.
In this study, we observed that only 6.5% of North-East Indian H. pylori strains were resistant for clarithromycin showing mutation at A2143G and T2182C positions of 23S rRNA gene. Resistance for levofloxacin was observed in 89.1% of the H. pylori strains showing mutations at asparagine to lysine at 87 and aspartic acid to glycine/tyrosine/asparagine at 91 positions of gyraseA gene. The phylogenetic tree of the partial sequence of 23S rRNA and gyraseA gene depicts that the North-East Indian strains falls in different cluster when compared to other countries.
Resistance for clarithromycin was less in North-East Indian strains but high for levofloxacin indicating that first-line therapy may be best and effective for eradication of H. pylori in this region. This study is the first report that showed antibiotic susceptibility pattern for clarithromycin and levofloxacin by mutation analysis. By partial sequencing of 23s rRNA and gyraseA gene, we found that North-East Indian strains are geographically distinct.
幽门螺杆菌可引发多种胃肠道疾病。对治疗幽门螺杆菌感染常用抗生素的耐药性是治疗失败的主要原因。本研究旨在确定克拉霉素和左氧氟沙星的抗菌药敏模式，并通过系统发育分析找出幽门螺杆菌23S rRNA和gyraseA基因部分序列的进化关系。
共检测46株幽门螺杆菌对克拉霉素和左氧氟沙星的药敏模式，并使用PhyML软件重建系统发育树。
在本研究中，我们观察到印度东北部的幽门螺杆菌菌株中只有6.5%对克拉霉素耐药，其23S rRNA基因的A2143G和T2182C位置发生了突变。在89.1%的幽门螺杆菌菌株中观察到对左氧氟沙星的耐药性，这些菌株gyraseA基因的第87位天冬酰胺突变为赖氨酸，第91位天冬氨酸突变为甘氨酸/酪氨酸/天冬酰胺。23S rRNA和gyraseA基因部分序列的系统发育树表明，与其他国家相比，印度东北部的菌株属于不同的聚类。
印度东北部菌株对克拉霉素的耐药性较低，但对左氧氟沙星的耐药性较高，这表明一线治疗可能是该地区根除幽门螺杆菌的最佳且有效的方法。本研究是首次通过突变分析显示克拉霉素和左氧氟沙星抗生素药敏模式的报告。通过对23s rRNA和gyraseA基因进行部分测序，我们发现印度东北部的菌株在地理上具有独特性。
