Schaffar N, Kessler J P, Bosler O, Jean A
Département de Physiologie et Neurophysiologie, CNRS UA 205, Faculté des Sciences et Techniques St Jérôme, Marseille, France.
Neuroscience. 1988 Sep;26(3):951-8. doi: 10.1016/0306-4522(88)90111-x.
Projections from several brainstem serotonergic nuclei to the nucleus tractus solitarii were investigated in the rat. Experiments were performed using a double labeling method combining retrograde radioautographic tracing and serotonin immunohistochemistry. After injection of the radioactive tracer ([3H] wheat germ agglutinin) into the lateral nucleus tractus solitarii, nerve cell bodies exhibiting both radioautographic labeling and immunostaining were detected in all the serotonergic nuclei investigated, namely the nucleus raphe magnus, the ventromedial paragigantocellular nucleus, the nuclei raphe pontis, medianus and dorsalis, the medial lemniscus and the reticulotegmental nucleus of the pons. Most of the double labeled perikarya observed were in the nucleus raphe magnus, the adjacent part of the paragigantocellular nucleus and the nucleus raphe dorsalis. Nerve cell bodies retrogradely labeled but devoid of immunostaining were also observed, together with the double labeled perikarya, within serotonergic nuclei. These results provide direct evidence that brainstem serotonergic neurons contribute to the innervation of the nucleus tractus solitarii. They indicate that the nucleus raphe magnus and the nucleus raphe dorsalis constitute two major sources of central serotonergic projections to the nucleus tractus solitarii.
在大鼠中研究了几个脑干5-羟色胺能核向孤束核的投射。实验采用逆行放射自显影示踪和5-羟色胺免疫组织化学相结合的双重标记方法进行。将放射性示踪剂（[3H]麦胚凝集素）注入孤束外侧核后，在所有研究的5-羟色胺能核中均检测到同时呈现放射自显影标记和免疫染色的神经细胞体，即中缝大核、腹内侧巨细胞旁核、中缝脑桥核、中缝正中核、中缝背核、内侧丘系和脑桥网状被盖核。观察到的大多数双重标记核周体位于中缝大核、巨细胞旁核的相邻部分和中缝背核。在5-羟色胺能核内，除了双重标记核周体外，还观察到逆行标记但无免疫染色的神经细胞体。这些结果提供了直接证据，证明脑干5-羟色胺能神经元参与孤束核的神经支配。它们表明，中缝大核和中缝背核构成了向孤束核投射的中枢5-羟色胺能的两个主要来源。
