Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin, 300071, China.
State Key Laboratory of Grassland Agro-ecosystems, Center for Grassland Microbiome, Lanzhou University, Lanzhou, 730000, China.
Bull Environ Contam Toxicol. 2020 Mar;104(3):333-338. doi: 10.1007/s00128-020-02793-1. Epub 2020 Jan 31.
Biochar has become a popular soil amendment. However, its effect on soil microbial community is still unclear. In the present study, maize straw biochar was pyrolysed at 300°C, 450°C and 600°C, respectively, and then was added to agricultural soil at the ratio of 0.5%, 1% and 2%. Bacterial dynamics was analyzed in the pot experiments using denaturing gradient gel electrophoresis. The results indicated that the pyrolysis temperature has great impact on the elemental composition, pH and porous structures of biochar. Moreover, pyrolysis temperature was primary factor to drive the variation of bacterial community structure in biochar amended soil. In addition, the results suggested that biochar amendments on agricultural soil would decrease the bacterial diversity, and selectively promote growth of functional bacteria to become the dominant community, which could increase the bacterial community organization and improve the stability of bacteria to counteract effects of perturbation.
生物炭已成为一种流行的土壤改良剂。然而，其对土壤微生物群落的影响尚不清楚。在本研究中，分别在 300°C、450°C 和 600°C 下热解玉米秸秆生物炭，然后以 0.5%、1%和 2%的比例添加到农业土壤中。通过变性梯度凝胶电泳在盆栽实验中分析细菌动态。结果表明，热解温度对生物炭的元素组成、pH 值和多孔结构有很大影响。此外，热解温度是驱动生物炭添加土壤中细菌群落结构变化的主要因素。此外，结果表明，农业土壤中添加生物炭会降低细菌多样性，并选择性地促进功能细菌的生长成为优势群落，这可以增加细菌群落的组织性，并提高细菌的稳定性，以抵消干扰的影响。
