Department of Cancer Immunology, Institute for Cancer Research, Oslo University Hospital-Radiumhospitalet, Oslo, Norway.
Methods Mol Biol. 2020;2115:119-131. doi: 10.1007/978-1-0716-0290-4_7.
Effective RNA delivery strategies for primary human monocytes and dendritic cells (DCs) are useful tools for both basic research and cancer immunotherapy applications. Compared to viral delivery, electroporation is a relatively safe and simple technique that has been established for most immune cells. This chapter describes the feasibility of introducing small interfering RNAs into human primary monocytes and DCs using either nucleofection or standard electroporation techniques. DC cancer vaccines that integrate siRNA targeting relevant DC-intrinsic immunosuppressive signals induced robust and durable anti-tumor immune responses.
有效的 RNA 递药策略对于原代人类单核细胞和树突状细胞（DC）而言是基础研究和癌症免疫治疗应用的有用工具。与病毒递送相比，电穿孔是一种相对安全和简单的技术，已被确立用于大多数免疫细胞。本章描述了使用核转染或标准电穿孔技术将小干扰 RNA 导入人原代单核细胞和 DC 的可行性。整合针对相关 DC 内在免疫抑制信号的 siRNA 的 DC 癌症疫苗可诱导强大且持久的抗肿瘤免疫反应。
