Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Nonoichi, 921-8836, Ishikawa, Japan.
Anal Biochem. 2020 Mar 15;593:113607. doi: 10.1016/j.ab.2020.113607. Epub 2020 Feb 5.
Quantification of polyamines, including putrescine, is generally performed using high-performance liquid chromatography (HPLC) or gas chromatography. However, these methods are time-consuming because of sample derivatization and analytical reagent preparation. In this study, we developed a simple and high-throughput putrescine quantification method on a 96-well microtiter plate using putrescine oxidase from Rhodococcus erythropolis NCIMB 11540, peroxidase, 4-aminoantipyrine, and N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt. The developed method (named as PuO-POD-4AA-TOPS method) was applicable to bacterial culture supernatants. Furthermore, putrescine concentrations determined by the developed method roughly corresponded to the concentrations determined by HPLC.
多胺（包括腐胺）的定量分析通常采用高效液相色谱（HPLC）或气相色谱法。但是，由于样品衍生化和分析试剂的制备，这些方法耗时较长。在本研究中，我们使用从红球菌 NCIMB 11540 中提取的腐胺氧化酶、过氧化物酶、4-氨基安替比林和 N-乙基-N-(3-磺丙基)-3-甲基苯胺钠盐，在 96 孔微量滴定板上开发了一种简单、高通量的腐胺定量方法。该方法（命名为 PuO-POD-4AA-TOPS 方法）适用于细菌培养液上清液。此外，该方法测定的腐胺浓度与 HPLC 测定的浓度大致相符。
