Laboratory of Immunobiology of Infections, Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, 93-232, Łódź, Poland.
Institute of Tuberculosis and Lung Diseases, Plocka 26, 01-138, Warsaw, Poland.
Immunobiology. 2020 May;225(3):151905. doi: 10.1016/j.imbio.2020.151905. Epub 2020 Jan 22.
We conducted a prospective study of 453 Polish patients suffering from pulmonary tuberculosis and 267 healthy controls. Selected polymorphisms of the genes encoding for collectins, ficolins and MBL-associated serine protease 2 were investigated as were serum concentrations of mannose-binding lectin, surfactant protein D, ficolin-1 and ficolin-3. The number of MBL2 gene exon 1 variant allele carriers was significantly higher in patients, compared with controls. The homozygosity for SFTPA2 +26 C > A SNP variant allele occurred less commonly within TB, while homozygosity for the FCN1 -542 G > A major allele was less frequent within the control group. Two patients were found MASP-2-deficient. Serum concentrations of MBL, SP-D and ficolin-1 were higher amongst patients while the converse was found for ficolin-3. Ficolin-1 had high specificity to differentiate between individuals with tuberculosis and healthy persons and therefore may be considered potential disease marker.
我们对 453 名患有肺结核的波兰患者和 267 名健康对照者进行了前瞻性研究。研究了编码凝集素、纤维胶凝素和 MBL 相关丝氨酸蛋白酶 2 的基因的选定多态性，以及甘露聚糖结合凝集素、表面活性剂蛋白 D、ficolin-1 和 ficolin-3 的血清浓度。与对照组相比，MBL2 基因外显子 1 变异等位基因携带者在患者中的数量明显更高。SFTPA2 +26 C > A SNP 变异等位基因纯合子在 TB 中较少发生，而 FCN1 -542 G > A 主要等位基因在对照组中较少发生。发现有两名患者缺乏 MASP-2。患者的 MBL、SP-D 和 ficolin-1 血清浓度较高，而 ficolin-3 则相反。ficolin-1 对区分结核患者和健康人具有很高的特异性，因此可能被视为潜在的疾病标志物。
