Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, College of Environment, Hohai University, Nanjing 210098, China.
Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, College of Environment, Hohai University, Nanjing 210098, China.
Sci Total Environ. 2020 May 1;715:136950. doi: 10.1016/j.scitotenv.2020.136950. Epub 2020 Jan 25.
Microbes in epiphytic biofilms and surface sediments play crucial roles in the biogeochemical cycles in wetlands. However, little is known about the compositions of microbial community in wetlands dominated with submersed macrophytes. In this study, bacterial and eukaryotic community in epiphytic biofilms and surface sediments were investigated in wetlands with artificial plants and Myriophyllum verticillatum from September (27 °C) to January (9 °C). A total of 30 (including 13 bacterial and 17 eukaryotic) and 34 (including 14 bacterial and 20 eukaryotic) phyla were detected in epiphytic biofilms and sediments, respectively. Microbial community in epiphytic biofilms shifted with decreasing temperature, and biofilms on M. verticillatum were generally similar to those on artificial plants. Though the OTUs and Shannon values were significantly higher in sediments than epiphytic biofilms (p < 0.05), numbers of strongly correlated edges detected in biofilms (64 nodes with 182 edges) were at least three times of those in sediments (40 nodes with 57 edges) as revealed by co-occurrence networks analysis (|r| > 0.7, p < 0.05). These data suggest that there were complex interactions among microbes in epiphytic biofilms than sediments. Positive relationships among microbes revealed the predation, symbiosis, parasitism relationships and the collective degradation of organic matter, while negative ones may be ascribed to their different lifestyles. These results highlight that artificial plants play a similar role as submersed macrophytes as microbial carriers and can be potentially used an alternative substitutes to submersed macrophytes in wetlands.
附生生物膜和表层沉积物中的微生物在湿地的生物地球化学循环中起着至关重要的作用。然而，对于以沉水植物为主的湿地中微生物群落的组成，人们知之甚少。本研究调查了人工植物和菹草湿地中附生生物膜和表层沉积物中的细菌和真核生物群落。研究于 2019 年 9 月（27°C）至 2020 年 1 月（9°C）进行。在附生生物膜和沉积物中分别检测到 30 个（包括 13 个细菌和 17 个真核生物）和 34 个（包括 14 个细菌和 20 个真核生物）门。随着温度的降低，附生生物膜中的微生物群落发生了变化，菹草上的生物膜通常与人工植物上的生物膜相似。尽管沉积物中的 OTUs 和 Shannon 值明显高于附生生物膜（p<0.05），但共现网络分析显示，附生生物膜中检测到的强相关边缘数（64 个节点 182 个边缘）至少是沉积物中的三倍（40 个节点 57 个边缘）（|r|>0.7，p<0.05）。这些数据表明，附生生物膜中的微生物之间存在复杂的相互作用，而不是沉积物。微生物之间的正相关关系揭示了捕食、共生、寄生关系以及有机物的集体降解，而负相关关系可能归因于它们不同的生活方式。这些结果强调了人工植物作为微生物载体，可以发挥与沉水植物相似的作用，并可能成为湿地中沉水植物的替代物。
