Hellenic Agricultural Organisation-DEMETER, Veterinary Research Institute of Thessaloniki, Campus of Thermi, 570 01, Thermi, Greece.
School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Curr Microbiol. 2020 Jun;77(6):1089-1096. doi: 10.1007/s00284-020-01905-8. Epub 2020 Feb 1.
Lactococcosis is a disease encountered in a wide variety of fish species causing mortalities and having great economic impact on farmed fish. In this study, we report for the first time the isolation of a strain of the recently described novel species Lactococcus petauri, from rainbow trout suffering from lactococcosis. The aim of this study was to determine the complete genome sequence of L. petauri strain LG_SAV_20 and to characterize its antimicrobial resistance and virulence. The genome of L. petauri LG_SAV_20 consists of 2,078,949 base pair (bp) with a GC content of 38.05%, 1950 predicted coding sequence (CDS), and 60 RNAs (51 tRNAs, 3 ncRNAs, and 6 rRNAs). Phylogenetic analysis revealed that L. petauri LG_SAV_20 shares most of its genome with L. garvieae strains isolated from rainbow trout. Detection of genes associated with antimicrobial resistance indicated that the isolate possesses the multidrug transporter mdt(A) gene, while using comparative analysis we identified several genes that might be related to bacterial pathogenesis. This genomic information provides new insights into the role of this novel species as an etiological agent of lactococcosis.
乳球菌病是一种广泛存在于各种鱼类中的疾病，可导致鱼类死亡，并对养殖鱼类造成巨大的经济影响。在本研究中，我们首次报道了从患有乳球菌病的虹鳟鱼中分离到一种新型物种乳球菌巴氏亚种（Lactococcus petauri）的菌株。本研究的目的是确定 L. petauri 菌株 LG_SAV_20 的全基因组序列，并对其抗菌药物耐药性和毒力进行表征。L. petauri LG_SAV_20 的基因组由 2,078,949 个碱基对（bp）组成，GC 含量为 38.05%，预测有 1950 个编码序列（CDS）和 60 个 RNA（51 个 tRNA、3 个非编码 RNA 和 6 个 rRNA）。系统发育分析表明，L. petauri LG_SAV_20 的基因组与从虹鳟鱼中分离到的 L. garvieae 菌株有很大的相似性。对与抗菌药物耐药性相关的基因的检测表明，该分离株携带多药转运蛋白 mdt(A)基因，而通过比较分析，我们鉴定出了几个可能与细菌发病机制相关的基因。这些基因组信息为该新型物种作为乳球菌病的病原体的作用提供了新的见解。
