Ji Jia-Heng, Zhou Lei, Mbadinga Serge Maurice, Irfan Muhammad, Liu Yi-Fan, Pan Pan, Qi Zhen-Zhen, Chen Jing, Liu Jin-Feng, Yang Shi-Zhong, Gu Ji-Dong, Mu Bo-Zhong
State Key Laboratory of Bioreactor Engineering and School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People's Republic of China.
Department of Chemical, Polymer and Composite Materials Engineering, University of Engineering and Technology, KSK Campus, Lahore, 54890, Pakistan.
AMB Express. 2020 Feb 1;10(1):23. doi: 10.1186/s13568-020-0956-5.
In the present study, a methanogenic alkane-degrading (a mixture of C to Cn-alkanes) culture enriched from production water of a low-temperature oil reservoir was established and assessed. Significant methane production was detected in the alkane-amended enrichment cultures compared with alkane-free controls over an incubation period of 1 year. At the end of the incubation, fumarate addition metabolites (C to C alkylsuccinates) and assA genes (encoding the alpha subunit of alkylsuccinate synthase) were detected only in the alkane-amended enrichment cultures. Microbial community analysis showed that putative syntrophic n-alkane degraders (Smithella) capable of initiating n-alkanes by fumarate addition mechanism were enriched in the alkane-amended enrichment cultures. In addition, both hydrogenotrophic (Methanocalculus) and acetoclastic (Methanothrix) methanogens were also observed. Our results provide further evidence that alkanes can be activated by addition to fumarate under methanogenic conditions.
在本研究中，建立并评估了一种从低温油藏采出水中富集的产甲烷烷烃降解菌（C 到 Cn 链烷烃的混合物）培养物。在为期 1 年的培养期内，与不含烷烃的对照相比，在添加烷烃的富集培养物中检测到显著的甲烷产生。培养结束时，仅在添加烷烃的富集培养物中检测到富马酸酯添加代谢物（C 到 C 烷基琥珀酸酯）和 assA 基因（编码烷基琥珀酸合酶的α亚基）。微生物群落分析表明，能够通过富马酸酯添加机制启动正构烷烃降解的假定互营正构烷烃降解菌（史密斯氏菌属）在添加烷烃的富集培养物中得到富集。此外，还观察到了氢营养型（甲烷球菌属）和乙酸营养型（甲烷丝状菌属）产甲烷菌。我们的结果提供了进一步的证据，表明在产甲烷条件下，烷烃可以通过添加到富马酸酯中而被激活。
