Department of Agricultural Sciences, Biotechnology and Food Sciences, Cyprus University of Technology, P.O. Box 50329, Lemesos, Cyprus.
Department of Animal and Veterinary Sciences, Scotland's Rural College, EH25 9RG, Midlothian, UK.
J Dairy Res. 2020 Feb;87(1):23-26. doi: 10.1017/S0022029919000992. Epub 2020 Feb 3.
The objective of the research reported in this research communication was the identification and association of single nucleotide polymorphisms (SNP) in the ovine DGAT1, FASN, SCD1 and ACAA2 genes with milk fat percentage and fatty acid (FA) content. Three consecutive monthly milk samplings were obtained from a total of 429 purebred Chios ewes during mid-lactation. Genotypic data were jointly analyzed with 1184 fat content and 37 718 FA percentage records using mixed models. The 3' untranslated region (UTR) of the DGAT1 gene and the 5' and 3'UTRs of the SCD1 gene appeared to be monomorphic. The FASN g.14777C>T SNP on exon 31 was associated with C13:0 and the ACAA2 g.2982T>C SNP on the 3'UTR was associated with C9:0, C11:0, C12:1 cis-9, C13:0 and the ω6/ω3 index, while fat percentage was not affected by the identified SNPs. The results could be useful for breeding programs aiming to improve the quality and nutritional value of ovine milk.
本研究通讯报道的研究目的是鉴定和关联绵羊 DGAT1、FASN、SCD1 和 ACAA2 基因中的单核苷酸多态性（SNP）与乳脂百分比和脂肪酸（FA）含量的关系。在泌乳中期，从总共 429 只纯种基耶罗斯母羊中连续三次每月采集一次奶样。使用混合模型，将基因型数据与 1184 个脂肪含量和 37718 个 FA 百分比记录联合进行分析。DGAT1 基因的 3'非翻译区（UTR）和 SCD1 基因的 5'和 3'UTR 似乎呈单态性。外显子 31 上的 FASN g.14777C>T SNP 与 C13:0 相关，3'UTR 上的 ACAA2 g.2982T>C SNP 与 C9:0、C11:0、C12:1cis-9、C13:0 和 ω6/ω3 指数相关，而脂肪百分比不受鉴定 SNP 的影响。这些结果可能对旨在提高绵羊奶质量和营养价值的育种计划有用。
