Kalmykova Natalia V, Shcherbanyuk Anna V, Moiseev Sergei I, Bichkova Natalia V, Davidova Natalia I, Samoilova Kira A
The Nikiforov Russian Center of Emergency and Radiation Medicine, St.Petersburg, Russia.
Institute of Cytology of the Russian Academy of Sciences, Saint-Petersburg, Russia.
Laser Ther. 2019 Sep 30;28(3):164-170. doi: 10.5978/islsm.28_19-OR-12.
Polychromatic light is actively used in medicine. However, its oncological safety and effect against cytotoxic therapy remains poorly studied. Multiple myeloma (MM) develops in the bone marrow and therefore malignant plasma cells are inaccessible to direct exposure to light. The aim of our work was to study the influence of polychromatic visible and infra-red light (pVIS + pIR) on growth and sensitivity of the myeloma cells to the cytotoxic effect of drug bortezomib (BTZ) through systemic mechanism.
We explored the effect of volunteers blood sera after their 4-daily irradiations with pVIS + pIR light (480-3400 nm) on growth and viability of the human myeloma RPMI 8226 cells in the presence of BTZ at its application in 2 modes: short-term (1 h, 300 nM) and long-term ( 96 h, 5 nM). Viability and proliferative activity of cells was evaluated by MTT assay.
It was found that photomodified blood sera delayed growth of myeloma RPMI 8226 cells (by 25 % in 48 h and 23 % in 96 h) but had no effect on spontaneous and mitogen-induced proliferation of autologous peripheral blood T- and B-lymphocytes. We also revealed that pVIS + pIR did not change RPMI 8226 cells sensitivity to BTZ.
The results suggest the systemic mechanism of polychromatic light and argue in favor of its oncological safety during/after BTZ therapy of MM patients without effect on the drug cytotoxicity.
多色光在医学中得到了广泛应用。然而，其肿瘤学安全性以及对细胞毒性疗法的影响仍研究不足。多发性骨髓瘤（MM）在骨髓中发生，因此恶性浆细胞无法直接暴露于光线下。我们研究的目的是通过全身机制研究多色可见光和红外光（pVIS + pIR）对骨髓瘤细胞生长以及对硼替佐米（BTZ）细胞毒性作用敏感性的影响。
我们探究了志愿者血液血清在接受4天的pVIS + pIR光（480 - 3400 nm）照射后，在两种BTZ应用模式下（短期（1小时，300 nM）和长期（96小时，5 nM））对人骨髓瘤RPMI 8226细胞生长和活力的影响。通过MTT法评估细胞的活力和增殖活性。
发现光修饰的血液血清延迟了骨髓瘤RPMI 8226细胞的生长（48小时延迟25%，96小时延迟23%），但对自体外周血T和B淋巴细胞的自发增殖和有丝分裂原诱导的增殖没有影响。我们还发现pVIS + pIR并未改变RPMI 8226细胞对BTZ的敏感性。
结果表明多色光的全身机制，并支持其在MM患者BTZ治疗期间/之后的肿瘤学安全性，且对药物细胞毒性无影响。
