Protective Allele for Multiple Sclerosis HLA-DRB1*01:01 Provides Kinetic Discrimination of Myelin and Exogenous Antigenic Peptides.

Risk of the development of multiple sclerosis (MS) is known to be increased in individuals bearing distinct class II human leukocyte antigen (HLA) variants, whereas some of them may have a protective effect. Here we analyzed distribution of a highly polymorphous HLA- locus in more than one thousand relapsing-remitting MS patients and healthy individuals of Russian ethnicity. Carriage of HLA15 and HLA03 alleles was associated with MS risk, whereas carriage of HLA01 and HLA11 was found to be protective. Analysis of genotypes revealed the compensatory effect of risk and resistance alleles . We have identified previously unknown MBP peptide located at the C-terminus of MBP protein and MBP peptide that bound to recombinant HLA-DRB101:01 protein with affinity comparable to that of classical antigenic peptide 306-318 from the hemagglutinin (HA) of the influenza virus demonstrating the ability of HLA-DRB101:01 to present newly identified MBP and MBP peptides. Measurements of kinetic parameters of MBP and HA peptides binding to HLA-DRB101:01 catalyzed by HLA-DM revealed a significantly lower rate of CLIP exchange for MBP and MBP peptides as opposed to HA peptide. Analysis of the binding of chimeric MBP-HA peptides demonstrated that the observed difference between MBP, MBP, and HA peptide epitopes is caused by the lack of anchor residues in the C-terminal part of the MBP peptides resulting in a moderate occupation of P6/7 and P9 pockets of HLA-DRB101:01 by MBP and MBP peptides in contrast to HA peptide. This leads to the P1 and P4 docking failure and rapid peptide dissociation and release of empty HLA-DM-HLA-DR complex. We would like to propose that protective properties of the HLA01 allele could be directly linked to the ability of HLA-DRB101:01 to kinetically discriminate between antigenic exogenous peptides and endogenous MBP derived peptides.