Wang Yaxi, Feng Tianying, Duan Shasha, Shi Yilu, Li Shuling, Zhang Xiaoshan, Zhang Lei
Department of Ultrasound, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010050, P.R. China.
Department of Medical Ultrasound, Bao'an Central Hospital of Shenzhen, Shenzhen, Guangdong 518102, P.R. China.
Exp Ther Med. 2020 Feb;19(2):1288-1296. doi: 10.3892/etm.2019.8330. Epub 2019 Dec 16.
The present study aimed to explore the expression and effects of microRNA (miR)-155 in synovial fibroblasts of patients with rheumatoid arthritis (RA). A total of 89 synovial tissues from RA patients and 49 control synovial tissues were collected, and the levels of miR-155 were measured by reverse transcription quantitative-PCR and western blotting. Fibroblast-like synoviocytes (FLS) were isolated from synovial tissues from the control group and were used to evaluate the roles of miR-155 and forkhead box protein O3a (FOXO3a). MTT assay was used to measure the proliferation of FLS. The expression of miR-155 in RA synovial tissues was significantly higher than that in the control group, but the expression of FOXO3a was significantly lower. In RA synovial tissues, miR-155 expression was negatively correlated with FOXO3a expression, but was positively correlated with the release of inflammatory cytokines interleukin (IL)-1β, IL-6 and tumor necrosis factor-α (TNF-α). A dual-luciferase reporter system showed that miR-155 inhibited the expression of FOXO3a in FLS cells. miR-155 also promoted secretion of the inflammatory cytokines IL-1β, IL-6 and TNF-α by FLS and proliferation of these cells by targeting FOXO3a.
本研究旨在探讨微小RNA（miR）-155在类风湿关节炎（RA）患者滑膜成纤维细胞中的表达及作用。共收集了89例RA患者的滑膜组织和49例对照滑膜组织，采用逆转录定量聚合酶链反应和蛋白质免疫印迹法检测miR-155水平。从对照组滑膜组织中分离出成纤维样滑膜细胞（FLS），用于评估miR-155和叉头框蛋白O3a（FOXO3a）的作用。采用MTT法检测FLS的增殖情况。RA滑膜组织中miR-155的表达显著高于对照组，但FOXO3a的表达显著低于对照组。在RA滑膜组织中，miR-155表达与FOXO3a表达呈负相关，但与炎性细胞因子白细胞介素（IL）-1β、IL-6和肿瘤坏死因子-α（TNF-α）的释放呈正相关。双荧光素酶报告系统显示，miR-155抑制FLS细胞中FOXO3a的表达。miR-155还通过靶向FOXO3a促进FLS分泌炎性细胞因子IL-1β、IL-6和TNF-α，并促进这些细胞的增殖。
