格列本脲/二甲双胍疗法对链脲佐菌素诱导的糖尿病大鼠骨生物力学退变的影响。
THE EFFECT OF GLUCOVANCE THERAPY ON BIOMECHANICAL DETERIORATION OF BONE IN STREPTOZOTOCIN-INDUCED DIABETIC RATS.
作者信息
Demirel C, Korkmaz H, Gurgul S, Yildiz A, Akarsu E, Erdal N
机构信息
Gaziantep University, Faculty of Medicine, Dept. of Biophysics, Gaziantep, Turkey.
Suleyman Demirel University, Faculty of Medicine, Dept. of Endocrinology and Metabolic Disease, Isparta, Mersin University, Faculty of Medicine, Mersin, Turkey.
出版信息
Acta Endocrinol (Buchar). 2019 Jul-Sep;15(3):301-304. doi: 10.4183/aeb.2019.301.
AIM
Is to evaluate the influence of glucovance therapy on biomechanical properties of bone in streptozotocin - induced diabetes mellitus (DM) in rats.
MATERIALS AND METHODS
A total of 28 male Wistar-Albino rats (12-week-old; 210-300 g) were divided into 4 groups including control (C; no treatment; n=7), sham [Sh; distilled water (gavage, for 8 weeks); n=7], diabetes [DM; streptozotocin (45 mg/kg, single i.p injection); n=7] and diabetes+ Glucovance treatment [DM+G; streptozotocin (45 mg/kg, single i.p injection) + Glucovance (Glucovance, 500/5 mg/kg/day/rat, gavage, for 8 weeks); n=7] groups. Body weight, blood glucose levels (BGLs), bone mineral density (BMD) and geometric/mechanical properties of bone tissue were evaluated. BGLs in diabetic rats were significantly increased compared to non-diabetic rats, while the body weights were decreased (p<0.05).
RESULTS
A significant difference was not detected between groups with regard to cross-sectional area of diaphyseal femur (p>0.05). Maximum load, energy absorption capacity, ultimate stress, ultimate strain, toughness and displacement were shown to decrease and stiffness was shown to increase in DM rats (p<0.05). Ultimate stress and maximum load were significantly increased in DM+G groups compared to DM groups (p<0.05).
CONCLUSION
Glucovance treatment seems to be effective in restoration of biomechanical deterioration of bone specific to STZ-induced DM.
目的
评估格列吡嗪复方制剂疗法对链脲佐菌素诱导的大鼠糖尿病（DM）骨生物力学特性的影响。
材料与方法
总共28只雄性Wistar白化大鼠（12周龄；体重210 - 300克）被分为4组，包括对照组（C；未治疗；n = 7）、假手术组[Sh；蒸馏水（灌胃，持续8周）；n = 7]、糖尿病组[DM；链脲佐菌素（45毫克/千克，单次腹腔注射）；n = 7]和糖尿病 + 格列吡嗪复方制剂治疗组[DM + G；链脲佐菌素（45毫克/千克，单次腹腔注射） + 格列吡嗪复方制剂（格列吡嗪复方制剂，500/5毫克/千克/天/只大鼠，灌胃，持续8周）；n = 7]。评估体重、血糖水平（BGLs）、骨矿物质密度（BMD）以及骨组织的几何/力学特性。与非糖尿病大鼠相比，糖尿病大鼠的血糖水平显著升高，而体重下降（p < 0.05）。
结果
在骨干股骨的横截面积方面，各实验组之间未检测到显著差异（p > 0.05）。糖尿病大鼠的最大负荷、能量吸收能力、极限应力、极限应变、韧性和位移均降低，而刚度增加（p < 0.05）。与糖尿病组相比，糖尿病 + 格列吡嗪复方制剂治疗组的极限应力和最大负荷显著增加（p < 0.05）。
结论
格列吡嗪复方制剂治疗似乎对恢复链脲佐菌素诱导的糖尿病特异性骨生物力学恶化有效。
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