Faculty of Biomedical Engineering, Fourth Military Medical University, 17 West Changle Road, Xi'an 710032, China.
Osteoporos Int. 2011 Jun;22(6):1885-95. doi: 10.1007/s00198-010-1447-3. Epub 2010 Oct 26.
The present study was the first report demonstrating that pulsed electromagnetic field (PEMF) could partially prevent bone strength and architecture deterioration and improve the impaired bone formation in streptozotocin-induced diabetic rats. The findings indicated that PEMF might become a potential additive method for inhibiting diabetic osteopenia or osteoporosis.
Diabetes mellitus (DM) can cause various musculoskeletal abnormalities. Optimal therapeutic methods for diabetic bone complication are still lacking. It is essential to develop more effective and safe therapeutic methods for diabetic bone disorders. Pulsed electromagnetic field (PEMF) as an alternative noninvasive method has proven to be effective for treating fracture healing and osteoporosis in non-diabetic conditions. However, the issue about the therapeutic effects of PEMF on diabetic bone complication has not been previously investigated.
We herein systematically evaluated the preventive effects of PEMF on diabetic bone loss in streptozotocin-treated rats. Two similar experiments were conducted. In each experiment, 16 diabetic and eight non-diabetic rats were equally assigned to the control, DM, and DM + PEMF group. DM + PEMF group was subjected to daily 8-h PEMF exposure for 8 weeks.
In experiment 1, three-point bending test suggested that PEMF improved the biomechanical quality of diabetic bone tissues, evidenced by increased maximum load, stiffness, and energy absorption. Microcomputed tomography analysis demonstrated that DM-induced bone architecture deterioration was partially reversed by PEMF, evidenced by increased Tb.N, Tb.Th, BV/TV, and Conn.D and reduced Tb.Sp and SMI. Serum OC analysis indicated that PEMF partially prevented DM-induced decrease in bone formation. In experiment 2, no significant difference in the bone resorption marker TRACP5b was observed. These biochemical findings were further supported by the dynamic bone histomorphometric parameters BFR/BS and Oc.N/BS.
The results demonstrated that PEMF could partially prevent DM-induced bone strength and architecture deterioration and improve the impaired bone formation. PEMF might become a potential additive method for inhibiting diabetic osteoporosis.
本研究首次报道了脉冲电磁场（PEMF）可部分预防骨强度和结构恶化，并改善链脲佐菌素诱导的糖尿病大鼠受损的骨形成。研究结果表明，PEMF 可能成为抑制糖尿病性骨质疏松症或骨质疏松症的一种潜在的附加方法。
糖尿病（DM）可引起多种骨骼肌肉异常。对于糖尿病性骨并发症的最佳治疗方法仍然缺乏。开发更有效和安全的治疗糖尿病性骨疾病的方法至关重要。脉冲电磁场（PEMF）作为一种替代的非侵入性方法，已被证明对治疗非糖尿病患者的骨折愈合和骨质疏松症有效。然而，关于 PEMF 对糖尿病性骨并发症的治疗效果的问题尚未被研究。
我们系统地评估了 PEMF 对链脲佐菌素处理的大鼠糖尿病性骨丢失的预防作用。进行了两项类似的实验。在每个实验中，将 16 只糖尿病大鼠和 8 只非糖尿病大鼠均等分配到对照组、DM 组和 DM+PEMF 组。DM+PEMF 组每天接受 8 小时的 PEMF 暴露，共 8 周。
在实验 1 中，三点弯曲试验表明，PEMF 改善了糖尿病骨组织的生物力学质量，表现为最大载荷、刚度和能量吸收的增加。微计算机断层扫描分析表明，PEMF 部分逆转了 DM 引起的骨结构恶化，表现为 Tb.N、Tb.Th、BV/TV 和 Conn.D 的增加以及 Tb.Sp 和 SMI 的减少。血清 OC 分析表明，PEMF 部分预防了 DM 引起的骨形成减少。在实验 2 中，未观察到骨吸收标志物 TRACP5b 的显著差异。这些生化发现进一步得到了动态骨组织形态计量学参数 BFR/BS 和 Oc.N/BS 的支持。
结果表明，PEMF 可部分预防 DM 引起的骨强度和结构恶化，并改善受损的骨形成。PEMF 可能成为抑制糖尿病性骨质疏松症的一种潜在的附加方法。
