Lanzhou University.
First Hospital of Lanzhou University.
Brief Bioinform. 2021 Jan 18;22(1):536-544. doi: 10.1093/bib/bbaa006.
Gastric cancer (GC) continues to be one of the major causes of cancer deaths worldwide. Meanwhile, liquid biopsies have received extensive attention in the screening and detection of cancer along with better understanding and clinical practice of biomarkers. In this work, 58 routine blood biochemical indices were tentatively used as integrated markers, which further expanded the scope of liquid biopsies and a discrimination system for GC consisting of 17 top-ranked indices, elaborated by random forest method was constructed to assist in preliminary assessment prior to histological and gastroscopic diagnosis based on the test data of a total of 2951 samples. The selected indices are composed of eight routine blood indices (MO%, IG#, IG%, EO%, P-LCR, RDW-SD, HCT and RDW-CV) and nine blood biochemical indices (TP, AMY, GLO, CK, CHO, CK-MB, TG, ALB and γ-GGT). The system presented a robust classification performance, which can quickly distinguish GC from other stomach diseases, different cancers and healthy people with sensitivity, specificity, total accuracy and area under the curve of 0.9067, 0.9216, 0.9138 and 0.9720 for the cross-validation set, respectively. Besides, this system can not only provide an innovative strategy to facilitate rapid and real-time GC identification, but also reveal the remote correlation between GC and these routine blood biochemical parameters, which helped to unravel the hidden association of these parameters with GC and serve as the basis for subsequent studies of the clinical value in prevention program and surveillance management for GC. The identification system, called GC discrimination, is now available online at http://lishuyan.lzu.edu.cn/GC/.
胃癌（GC）仍然是全球癌症死亡的主要原因之一。与此同时，随着对生物标志物的更好理解和临床实践，液体活检在癌症的筛查和检测中受到了广泛关注。在这项工作中，我们初步将 58 项常规血液生化指标用作综合标志物，这进一步扩大了液体活检的范围，并构建了一个由 17 个排名最高的指标组成的 GC 判别系统，该系统通过随机森林方法构建，用于根据总共 2951 个样本的测试数据，在组织学和胃镜诊断之前协助初步评估。所选指标由八项常规血液指标（MO%、IG#、IG%、EO%、P-LCR、RDW-SD、HCT 和 RDW-CV）和九项血液生化指标（TP、AMY、GLO、CK、CHO、CK-MB、TG、ALB 和γ-GGT）组成。该系统呈现出强大的分类性能，可以快速区分 GC 与其他胃病、不同癌症和健康人群，交叉验证集的灵敏度、特异性、总准确率和曲线下面积分别为 0.9067、0.9216、0.9138 和 0.9720。此外，该系统不仅提供了一种创新策略，以促进快速实时的 GC 识别，还揭示了 GC 与这些常规血液生化参数之间的远程相关性，有助于揭示这些参数与 GC 的隐藏关联，并为随后研究 GC 预防计划和监测管理的临床价值提供依据。该识别系统名为 GC 判别，现可在 http://lishuyan.lzu.edu.cn/GC/ 上获取。
