Animal Physiology Division, ICAR-National Institute of Animal Nutrition and Physiology, Bangalore, India.
Anim Biotechnol. 2021 Aug;32(4):470-478. doi: 10.1080/10495398.2020.1721513. Epub 2020 Feb 3.
The present study in sheep model was to find out the interaction of apoptotic transcripts, that is, and and pluripotency related transcripts, that is, and in ovine embryos produced at different O concentrations (20% and 5% O) to compare their developmental potential. Oxygen concentrations did not influence the maturation and cleavage rate but the percentage of morula and blastocysts was significantly more at 5% as compared to 20% O. A significant upregulated expression of and genes and significantly downregulated expression of and were observed in the blastocysts at 5% than those at 20% O. The expression of was not influenced by the O concentration. Among the pluripotency related transcripts, the expression of was significantly upregulated and the expression of and was significantly downregulated in embryos at 5% than at 20% O. The study concluded that the embryos produced at low O (5%) concentration regulate the expression of developmental genes related to apoptosis and pluripotency to improve the developmental potential of embryos as compared to high O (20%) concentration.
本研究在绵羊模型中，旨在找出凋亡相关转录本和多能性相关转录本在不同氧浓度（20%和 5%）下产生的绵羊胚胎中的相互作用，以比较它们的发育潜能。氧浓度不影响成熟和卵裂率，但与 20%相比，5%的桑葚胚和囊胚的百分比明显更高。在 5%的氧浓度下，与 20%的氧浓度相比，观察到 基因的表达显著上调，而 基因的表达显著下调。在多能性相关转录本中，与 20%的氧浓度相比，5%的氧浓度下，胚胎中 基因的表达显著上调，而 基因和 基因的表达显著下调。研究得出的结论是，与高氧（20%）浓度相比，低氧（5%）浓度下产生的胚胎调节与凋亡和多能性相关的发育基因的表达，以提高胚胎的发育潜能。
