The Witold Stefański Institute of Parasitology Polish Academy of Sciences, Warsaw, Poland.
BIOMIBO, Warsaw, Poland.
PLoS One. 2020 Feb 3;15(2):e0228407. doi: 10.1371/journal.pone.0228407. eCollection 2020.
Cell homeostasis requires the correct levels of reactive oxygen species (ROS) to be maintained as these regulate the proliferation and differentiation of cells, and control the immune response and inflammation. High levels of ROS can cause oxidative stress, leading to protein, lipid and DNA damage, or even cell death. Under physiological conditions, the rate of autophagy remains stable; however, it can be accelerated by a number of exogenous stimuli such as oxidative stress, starvation or hypoxia, leading to cell death. The present paper examines the effect of Conidiobolus coronatus infection on the immune response, oxidative stress processes and autophagy in the greater wax moth, Galleria mellonella. Fungal infection was found to result in the disorganization of the cytoskeleton of the larval immune cells and the enhancement of oxidative defense processes. Lipid peroxidation and autophagy were also induced in the hemocytes. Our findings show that G. mellonella is an ideal model for exploring immune mechanisms.
细胞内稳态需要维持适当水平的活性氧（ROS），因为 ROS 可以调节细胞的增殖和分化，并控制免疫反应和炎症。ROS 水平过高会导致氧化应激，导致蛋白质、脂质和 DNA 损伤，甚至细胞死亡。在生理条件下，自噬的速度保持稳定；然而，许多外源性刺激物，如氧化应激、饥饿或缺氧，可以加速自噬，导致细胞死亡。本文研究了冠突散囊菌感染对大蜡螟免疫反应、氧化应激过程和自噬的影响。研究发现，真菌感染导致幼虫免疫细胞的细胞骨架紊乱，并增强了氧化防御过程。血细胞中也诱导了脂质过氧化和自噬。我们的研究结果表明，大蜡螟是探索免疫机制的理想模型。
