Menta Lakshmi Naga Kavya, Law Soffi Kei Kei, Tan Hock Siew
School of Science, Monash University Malaysia, Subang Jaya, Malaysia.
J R Soc N Z. 2024 May 15;55(6):1610-1622. doi: 10.1080/03036758.2024.2353736. eCollection 2025.
Identifying promising probiotic candidates for further development remains challenging. Traditional mammalian models are invaluable for assessing efficacy, but the associated limitations slow the preliminary screening process. The larvae have emerged as a powerful screening model. Most probiotic studies on use intra-hemocelic injection, bypassing the natural gut entry point, making it analogous to introducing probiotics into the mammalian blood. Therefore, despite their advantages, discrepancies exist between and mammalian models, particularly regarding the route of probiotic administration. This study bridges this gap by investigating the differential effects of the commonly studied intra-hemocelic injection and the less common oral administration of a probiotic, Nissle 1917 (EcN1917), on its protective efficacy against a gastrointestinal pathogen, Typhimurium ATCC 14028 (ST14028). This study demonstrated that oral EcN1917 pre-treatment significantly increased survivability against ST14028 infection compared to the control group and alleviated the pathogen gut burden. Notably, injection pre-treatment decreased survivability. This discrepancy is attributed to the dual nature of probiotics, exhibiting beneficial effects in the gut but acting as pathogens in non-native locations like the hemolymph, concluding that the route of probiotic administration in significantly impacts the protective effects of probiotics.
识别有前景的益生菌候选物以进行进一步开发仍然具有挑战性。传统的哺乳动物模型对于评估功效非常重要，但相关的局限性减缓了初步筛选过程。幼虫已成为一种强大的筛选模型。大多数关于[未提及具体对象]的益生菌研究使用血腔注射，绕过了自然的肠道进入点，这类似于将益生菌引入哺乳动物血液中。因此，尽管它们具有优势，但[未提及具体对象]模型与哺乳动物模型之间存在差异，特别是在益生菌给药途径方面。本研究通过调查常用的血腔注射和较少见的益生菌Nissle 1917（EcN1917）口服给药对其针对胃肠道病原体鼠伤寒沙门氏菌ATCC 14028（ST14028）的保护功效的不同影响，弥合了这一差距。本研究表明，与对照组相比，口服EcN1917预处理显著提高了对ST14028感染的存活率，并减轻了病原体在肠道的负担。值得注意的是，注射预处理降低了存活率。这种差异归因于益生菌的双重性质，即在肠道中表现出有益作用，但在非天然部位（如血淋巴）中充当病原体，得出结论：[未提及具体对象]中益生菌的给药途径显著影响益生菌的保护作用。
