Martins C, Darrigade A-S, Jacquemin C, Barnetche T, Taieb A, Ezzedine K, Boniface K, Seneschal J
INSERM U1035, BMGIC, Immuno-Dermatology Team, University of Bordeaux, Bordeaux, France.
Department of Dermatology and Pediatric Dermatology, National Reference Center for Rare Skin Disorders, Hôpital Saint-André, Bordeaux, France.
Br J Dermatol. 2020 Nov;183(5):899-908. doi: 10.1111/bjd.18902. Epub 2020 Mar 9.
Vitiligo is a chronic inflammatory skin disorder characterized by the loss of melanocytes. While a T helper cell (Th)1/cytotoxic T cell (Tc)1-skewed immune response is now well demonstrated in vitiligo, recent data suggest that the T-cell component could be more complex, involving different combinatorial T-cell subsets.
To analyse the phenotype and function of circulating CD4 and CD8 memory T-cell subsets in patients with stable and active vitiligo, in comparison with patients with psoriasis and healthy controls.
This is a monocentric, prospective, descriptive and exploratory study. Multiparametric flow cytometry analyses were performed to evaluate the surface expression of homing and T-cell-subset markers together with intracellular cytokine production in peripheral blood mononuclear cells from 60 patients with vitiligo, 25 patients with psoriasis and 28 healthy donors.
Vitiligo peripheral blood circulating effector and central memory T cells expressed similar proportions of skin-homing markers. Decrease in the frequencies of circulating CD4 and CD8 Th1/Tc1, Th17/Tc17, and Th1/Th17 or Tc1/Tc17 effector memory T-cell subsets were observed in patients with vitiligo compared with healthy donors. Similar observations were made in psoriasis. In contrast, vitiligo circulating T cells showed a similar capacity for proinflammatory cytokine production compared with those in psoriasis and healthy controls.
The decreased frequencies of circulating Th1/Tc1, Th17/Tc17 and Th1/Th17-Tc1/Tc17 cells suggest a possible migration of these T-cell subsets into the skin of patients with vitiligo. These could be targeted to prevent flares of the disease. What is already known about this topic? Vitiligo is a chronic inflammatory skin disorder associated with the loss of melanocytes. Vitiligo is characterized by a T helper cell (Th)1/cytotoxic T cell (Tc)1-skewed immune response in the skin. What does this study add? A thorough analysis of the phenotype and function of circulating memory T cells suggests the migration of Th1/Tc1, Th17/Tc17 and Th1/Th17-Tc1/Tc17 cell subsets in the skin. What is the translational message? A better understanding of the different immune T-cell subsets involved in vitiligo could lead to better therapeutic options. Linked Comment: Matos. Br J Dermatol 2020; 183:803.
白癜风是一种以黑素细胞缺失为特征的慢性炎症性皮肤病。虽然目前已充分证明白癜风存在辅助性T细胞（Th）1/细胞毒性T细胞（Tc）1偏向的免疫反应，但最近的数据表明T细胞成分可能更为复杂，涉及不同组合的T细胞亚群。
分析稳定期和进展期白癜风患者循环CD4和CD8记忆T细胞亚群的表型和功能，并与银屑病患者和健康对照进行比较。
这是一项单中心、前瞻性、描述性和探索性研究。采用多参数流式细胞术分析60例白癜风患者、25例银屑病患者和28名健康供者外周血单个核细胞中归巢和T细胞亚群标志物的表面表达以及细胞内细胞因子的产生情况。
白癜风患者外周血循环效应性和中枢记忆T细胞表达皮肤归巢标志物的比例相似。与健康供者相比，白癜风患者循环CD4和CD8 Th1/Tc1、Th17/Tc17以及Th1/Th17或Tc1/Tc17效应性记忆T细胞亚群的频率降低。银屑病患者也有类似的观察结果。相比之下，白癜风循环T细胞与银屑病患者和健康对照相比，产生促炎细胞因子的能力相似。
循环Th1/Tc1、Th17/Tc17和Th1/Th17-Tc1/Tc17细胞频率降低表明这些T细胞亚群可能迁移至白癜风患者皮肤中。可针对这些细胞亚群预防疾病发作。关于该主题已知的信息有哪些？白癜风是一种与黑素细胞缺失相关的慢性炎症性皮肤病。白癜风的特征是皮肤中辅助性T细胞（Th）1/细胞毒性T细胞（Tc）1偏向的免疫反应。本研究增加了什么内容？对循环记忆T细胞的表型和功能进行全面分析表明Th1/Tc1、Th17/Tc17和Th1/Th17-Tc1/Tc17细胞亚群迁移至皮肤中。转化信息是什么？更好地了解参与白癜风的不同免疫T细胞亚群可能会带来更好的治疗选择。相关评论：Matos。《英国皮肤病学杂志》2020年；183:803。
