Namikawa R, Kaneshima H, Lieberman M, Weissman I L, McCune J M
Department of Pathology, Stanford University School of Medicine, CA 94305.
Science. 1988 Dec 23;242(4886):1684-6. doi: 10.1126/science.3201256.
SCID-hu mice with human fetal thymic or lymph node implants were inoculated with the cloned human immunodeficiency virus-1 isolate, HIV-1JR-CSF. In a time- and dose-dependent fashion, viral replication spread within the human lymphoid organs. Combination immunohistochemistry and in situ hybridization revealed only viral RNA transcripts in most infected cells, but some cells had both detectable viral transcripts and viral protein. Infected cells were always more apparent in the medulla than in the cortex of the thymus. These studies demonstrate that an acute infection of human lymphoid organs with HIV-1 can be followed in the SCID-hu mouse.
将含有人类胎儿胸腺或淋巴结植入物的重症联合免疫缺陷-人类（SCID-hu）小鼠接种克隆的人类免疫缺陷病毒1型分离株HIV-1JR-CSF。病毒复制以时间和剂量依赖的方式在人类淋巴器官内扩散。联合免疫组织化学和原位杂交显示，大多数感染细胞中仅存在病毒RNA转录本，但有些细胞同时具有可检测到的病毒转录本和病毒蛋白。感染细胞在胸腺髓质中总是比在皮质中更明显。这些研究表明，在SCID-hu小鼠中可以追踪HIV-1对人类淋巴器官的急性感染。
