芒果苷局部给药可促进链脲佐菌素-烟酰胺诱导的2型糖尿病雄性大鼠伤口愈合。
Topical administration of mangiferin promotes healing of the wound of streptozotocin-nicotinamide-induced type-2 diabetic male rats.
作者信息
Lwin Ohn Mar, Giribabu Nelli, Kilari Eswar Kumar, Salleh Naguib
机构信息
Department of Physiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Pharmacology Division, A.U. College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, India.
出版信息
J Dermatolog Treat. 2021 Dec;32(8):1039-1048. doi: 10.1080/09546634.2020.1721419. Epub 2020 Feb 3.
PURPOSE
This study identifies the potential use of mangiferin gel to promote wound healing in diabetes mellitus (DM).
MATERIALS AND METHODS
Male rats were rendered diabetes mellitus intraperitoneal injection of streptozotocin and nicotinamide. Following diabetes development, wound was created at the back of the neck. 1% and 2% mangiferin gel and 1% silver sulphurdiazine (SS) gel (positive control) were applied to the wound for twenty-one (21) days. Fasting blood glucose (FBG) levels were weekly monitored. At the end of the treatment, rats were sacrificed and wound was excised and subjected for histopathological and molecular biological analysis.
RESULTS
No changes to serum FBG levels was noted throughout the period of mangiferin treatment. Albeit, a significant decrease in the size of the wound with increased in the skin thickness of surrounding the wound were observed. Increased expression and distribution of EGF, FGF, TGF-β, VEGF, PI3K, MMP and Nrf2 and decreased expression and distribution of TNFα and NF-κB p65 were observed in diabetic wound treated with topical mangiferin.
CONCLUSIONS
Mangiferin has potential to be used as an agent to promote wound healing in diabetic condition.
目的
本研究确定芒果苷凝胶在促进糖尿病（DM）伤口愈合方面的潜在用途。
材料与方法
雄性大鼠通过腹腔注射链脲佐菌素和烟酰胺诱导糖尿病。糖尿病形成后，在大鼠颈部背部制造伤口。将1%和2%的芒果苷凝胶以及1%的磺胺嘧啶银（SS）凝胶（阳性对照）应用于伤口，持续21天。每周监测空腹血糖（FBG）水平。治疗结束时，处死大鼠，切除伤口并进行组织病理学和分子生物学分析。
结果
在芒果苷治疗期间，血清FBG水平无变化。然而，观察到伤口大小显著减小，伤口周围皮肤厚度增加。在用局部芒果苷治疗的糖尿病伤口中，观察到表皮生长因子（EGF）、成纤维细胞生长因子（FGF）、转化生长因子-β（TGF-β）、血管内皮生长因子（VEGF）、磷脂酰肌醇-3激酶（PI3K）、基质金属蛋白酶（MMP）和核因子E2相关因子2（Nrf2）的表达和分布增加，肿瘤坏死因子α（TNFα）和核因子κB p65（NF-κB p65）的表达和分布减少。
结论
芒果苷有潜力作为一种促进糖尿病伤口愈合的药物。
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