Zucker R M, Elstein K H, Easterling R E, Massaro E J
NSI, Environmental Sciences, Research Triangle Park, NC 27709.
Toxicology. 1988 Dec 16;53(1):69-78. doi: 10.1016/0300-483x(88)90237-5.
Flow cytometric analysis of the cell cycle is most effectively accomplished with membrane-/cytoplasm-free ("clean") nuclei. Non-ionic detergents (e.g. NP40 or Triton X-100) commonly are employed to solubilize cell membranes/cytoplasm to produce "clean" nuclei. Treatment of murine erythroleukemic cells (MELC) with tri-n-butyltin methoxide, cadmium acetate, zinc sulfate, or lead acetate alters the properties of the cell membrane/cytoplasm complex making it resistant to NP40 dissolution. On a molar basis, the organotin compound was more effective in inducing resistance to detergent-mediated dissolution than the inorganic metal compounds. Resistance to NP40-mediated dissolution was manifested as an increase in the flow cytometric parameters 90 degrees scatter and fluorescein isothiocyanate (FITC) fluorescence and was confirmed by light microscopy.
细胞周期的流式细胞术分析最有效地通过无膜/无细胞质（“纯净”）的细胞核来完成。通常使用非离子去污剂（如NP40或Triton X-100）来溶解细胞膜/细胞质以产生“纯净”的细胞核。用甲醇三丁基锡、醋酸镉、硫酸锌或醋酸铅处理小鼠红白血病细胞（MELC）会改变细胞膜/细胞质复合物的性质，使其对NP40溶解具有抗性。以摩尔为基础，有机锡化合物比无机金属化合物在诱导对去污剂介导的溶解的抗性方面更有效。对NP40介导的溶解的抗性表现为流式细胞术参数90度散射和异硫氰酸荧光素（FITC）荧光增加，并通过光学显微镜得到证实。
