Department of Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan
Department of Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.
Anticancer Res. 2020 Feb;40(2):743-749. doi: 10.21873/anticanres.14005.
BACKGROUND/AIM: The hepatic stellate cells (HSCs) have relationship to cancer progression. The aim of this study is to investigate the effect of HSCs and the role of IL-6/Stat3 pathway on hepatocellular carcinoma (HCC) progression.
HCCs were co-cultured with HSCs. The viability and migration ability of cancer cells were detected. Epithelial-mesenchymal transition (EMT) marker (E-cadherin), stem cell marker (CD44) and p-signal transducer and activator of transcription 3 (p-STAT3) of cancer cells were evaluated. Finally, interleukin-6 (IL-6) neutralization was performed.
Co-culture of HCCs with HSCs increased cancer cell viability and migration ability. EMT and stemness of cancer cells increased with HSCs. Following IL-6 neutralization, phospho-STAT3 activation, cancer cell viability and migration, as well as EMT, and stemness of cancer cells decreased.
HSCs promoted HCC progression through the IL-6/STAT3 pathway.
背景/目的：肝星状细胞（HSCs）与癌症进展有关。本研究旨在探讨 HSCs 的作用以及 IL-6/Stat3 通路在肝细胞癌（HCC）进展中的作用。
将 HCC 与 HSCs 共培养。检测癌细胞的活力和迁移能力。评估癌细胞的上皮-间充质转化（EMT）标志物（E-钙黏蛋白）、干细胞标志物（CD44）和 p-信号转导和转录激活因子 3（p-STAT3）。最后，进行白细胞介素 6（IL-6）中和。
HSCs 与 HCC 共培养可增加癌细胞的活力和迁移能力。HSCs 增加了癌细胞的 EMT 和干性。IL-6 中和后，磷酸化 Stat3 激活、癌细胞活力和迁移以及癌细胞的 EMT 和干性降低。
HSCs 通过 IL-6/Stat3 通路促进 HCC 进展。
