Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan
Graduate School of Science and Technology for Innovation, Yamaguchi University, Yamaguchi, Japan.
J Bacteriol. 2020 Mar 26;202(8). doi: 10.1128/JB.00553-19.
is an industrial strain used for the vinegar production. Many strains with different phenotypic characteristics have been isolated so far. To understand the genetic background underpinning these phenotypes, a comparative genomic analysis of strains was conducted. Based on bioinformatics and experimental results, we report the following. (i) The gene repertoire related to the respiratory chains showed that several horizontal gene transfer events occurred after the divergence of these strains, indicating that the respiratory chain in has the diversity to adapt to its environment. (ii) There is a clear difference in thermotolerance even between 12 closely related strains. NBRC 3279, NBRC 3284, and NBRC 3283, in particular, which have only 55 mutations in total, showed differences in thermotolerance. The Na/H antiporter gene was mutated in the thermosensitive NBRC 3279 and NBRC 3284 strains and not in the thermotolerant NBRC 3283 strain. The Na/H antiporter activity of the three strains and expression of gene from NBRC 3283 in the two thermosensitive strains showed that these mutations are critical for thermotolerance. These results suggested that horizontal gene transfer events and several mutations have affected the phenotypes of these closely related strains., an industrial vinegar-producing strain, exhibits diverse phenotypic differences such as respiratory activity related to acetic acid production, acetic acid resistance, or thermotolerance. In this study, we investigated the correlations between genome sequences and phenotypes among closely related strains. The gene repertoire related to the respiratory chains showed that the respiratory components of has a diversity caused by several horizontal gene transfers and mutations. In three closely related strains with clear differences in their thermotolerances, we found that the insertion or deletion that occurred in the Na/H antiporter gene is directly related to their thermotolerance. Our study suggests that a relatively quick mutation has occurred in the closely related due to its genetic instability and that this has largely affected its phenotype.
是一种用于生产醋的工业菌株。迄今为止，已经分离出许多具有不同表型特征的菌株。为了了解这些表型背后的遗传背景，对菌株进行了比较基因组分析。基于生物信息学和实验结果，我们报告如下。(i)与呼吸链相关的基因库表明，这些菌株分化后发生了几次水平基因转移事件，表明 具有适应其环境的多样性呼吸链。(ii)即使在 12 株密切相关的菌株之间，耐热性也有明显差异。NBRC 3279、NBRC 3284 和 NBRC 3283 总共只有 55 个突变，耐热性不同。在热敏 NBRC 3279 和 NBRC 3284 菌株中，Na/H 反向转运蛋白基因 发生了突变，而在耐热 NBRC 3283 菌株中则没有。三株菌的 Na/H 反向转运蛋白活性及 NBRC 3283 菌株中 基因的表达表明，这些突变对耐热性至关重要。这些结果表明，水平基因转移事件和几个突变影响了这些密切相关菌株的表型。 ，一种工业醋生产菌株，表现出多种表型差异，如与醋酸生产相关的呼吸活性、耐酸或耐热性。在这项研究中，我们调查了密切相关 菌株的基因组序列与表型之间的相关性。与呼吸链相关的基因库表明， 的呼吸成分由于几次水平基因转移和突变而具有多样性。在耐热性差异明显的三株密切相关的菌株中，我们发现发生在 Na/H 反向转运蛋白基因 中的插入或缺失与它们的耐热性直接相关。我们的研究表明，由于其遗传不稳定性，在密切相关的 中发生了相对较快的突变，这在很大程度上影响了它的表型。
