Percio Stefano, Rotundo Federica, Gandellini Paolo
Department of Applied Research and Technological Development, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, 20133, Italy.
Department of Biosciences, University of Milan, Milan, 20133, Italy.
Data Brief. 2020 Jan 16;29:105139. doi: 10.1016/j.dib.2020.105139. eCollection 2020 Apr.
Although the role of 205 has been widely elucidated, the function of its host gene () is yet to be clarified. We have recently investigated whether this gene is a simple endorsement for miRNA production or it may act independently, demonstrating its action as nuclear long noncoding RNA able to control basal-luminal differentiation in the human prostate context, thus deserving the reannotation as , Long Epithelial -interacting Differentiation-related RNA. Here, we describe the loss and gain of function approaches experimentally used to modulate expression, and the bioinformatic procedures employed to analyze microarray data in our published article "LEADeR role of miR-205 host gene as long noncoding RNA in prostate basal cell differentiation" [1]. The high reproducibility of replicates, the strong concordance with a validation technique, and the coherent behavior observed for differentially expression features, both in terms of single genes and deregulated pathways, not only support the quality of the data, but also endorse their potential reuse. Very relevant are the diverse silencing and overexpression strategies employed (all of which analyzed in multiple biologically independent replicates), which should allow other scientists to analyze our dataset for the specific purpose of their research, may it be the study of function as miRNA host gene, the investigation of its miRNA-independent biological role or again the dissection of sequence involvement in the mechanism of action of long noncoding RNAs, which is a hot topic in the field.
尽管miR-205的作用已得到广泛阐释，但其宿主基因（）的功能仍有待阐明。我们最近研究了该基因是仅仅支持miRNA的产生，还是可能独立发挥作用，结果表明它作为一种核长链非编码RNA，能够在人类前列腺环境中控制基底-腔面分化，因此值得重新注释为长上皮相互作用分化相关RNA（Long Epithelial -interacting Differentiation-related RNA，LEADeR）。在此，我们描述了在我们已发表的文章《miR-205宿主基因作为长链非编码RNA在前列腺基底细胞分化中的LEADeR作用》[1]中实验性用于调节LEADeR表达的功能缺失和功能获得方法，以及用于分析微阵列数据的生物信息学程序。重复实验的高重现性、与验证技术的高度一致性，以及在单个基因和失调通路方面观察到的差异表达特征的一致行为，不仅支持了数据的质量，也认可了其潜在的再利用价值。所采用的多种沉默和过表达策略（所有这些都在多个生物学独立重复中进行了分析）非常重要，这应该能让其他科学家出于他们研究的特定目的分析我们的数据集，无论是研究其作为miRNA宿主基因的功能、探究其不依赖miRNA的生物学作用，还是再次剖析其序列在长链非编码RNA作用机制中的参与情况，这都是该领域的一个热门话题。
