微妙的序列变化改变了 RNA 适体 Broccoli 中的三联复合物动力学和 G-四链体动力学。
Subtle sequence variations alter tripartite complex kinetics and G-quadruplex dynamics in RNA aptamer Broccoli.
机构信息
Department of Chemical Physiology & Biochemistry, Oregon Health & Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, USA.
出版信息
Chem Commun (Camb). 2020 Feb 28;56(17):2634-2637. doi: 10.1039/c9cc09375c. Epub 2020 Feb 4.
Though extensively utilized, the fluorescent RNA aptamer Broccoli is poorly characterized with an unknown structure. Spectroscopic and kinetic investigations of tripartite complex formation reveal surprising differences between Broccoli and Spinach aptamers despite extreme sequence conservation. Our studies highlight how subtle sequence variations impart functional consequences of G-quadruplex-cation interactions in RNA.
尽管荧光 RNA 适体 Broccoli 被广泛应用，但它的特性却知之甚少，其结构未知。尽管序列高度保守，但对三分体复合物形成的光谱和动力学研究揭示了 Broccoli 和 Spinach 适体之间令人惊讶的差异。我们的研究强调了微小的序列变化如何赋予 RNA 中 G-四联体-阳离子相互作用的功能后果。
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