Molecular weight distribution of pretreated thickened waste activated sludge and fat, oil, and grease.

Co-digestion samples containing thickened waste activated sludge and fat, oil and grease were subjected to three different pretreatment methods, i.e., microwave at 175 °C, hyper-thermophilic stage at 70 °C, and conventional heat at 70 °C. The soluble matter extracted from the un-pretreated and pretreated samples were subjected to an ultrafiltration (UF) process using four different membrane sizes (300, 100, 10, and 1 kDa) for molecular weight distribution analysis. Every pretreatment method had a different effect on the solubilization and redistribution of the soluble matter (SCOD and TVFA). For example while MW pretreatment resulted in a significant increase in the SCOD at the lowest molecular weight (< 1 kDa) and at the highest molecular weight (> 300 kDa), Hyper pretreatment caused the majority of the SCOD ( ̴ 62.7% of total SCOD) to be concentrated at the smaller molecular weight range (< 10 kDa). The MW and hyper-thermophilic pretreatments were much more effective in increasing samples solubilization and biogas production compared to the conventional heat pretreatment. The hyper-thermophilic samples had the maximum improvement in cumulative biogas production from all the molecular weights compared to MW- and Heat-pretreated samples; Hyper-pretreated samples achieved 86.5% higher cumulative biogas production compared to the control.