Kaminota Nao, Ogawa Emiyu, Kumagai Hiroshi, Tsukada Kosuke, Arai Tsunenori
School of Fundamental Science and Technology, Graduate School of Science and Technology, Keio University, 3-14-1, Hiyoshi, Kohoku-ku, Yokohama, Kanagawa, 223-8522, Japan.
Department of Medical Engineering and Technology, School of Allied Health Science, Kitasato University, 1-15-1, Kitasato, Minami-ku, Sagamihara, Kanagawa, 252-0373, Japan.
Cardiovasc Eng Technol. 2020 Jun;11(3):308-315. doi: 10.1007/s13239-020-00458-y. Epub 2020 Feb 3.
A treatment device without a stent is needed for peripheral stenotic artery treatment. We have proposed short-term heating balloon angioplasty, photo-thermo dynamic balloon angioplasty (PTDBA). Though smooth muscle cells (SMCs) after PTDBA are fixed in a stretched formation in a porcine model, influences of this stimulus on SMCs have not been investigated. SMC migration after vascular dilatation would be related to chronic restenosis. The aim of this study was to examine respiratory activity and recovery ability of SMCs after short-term heating/stretch-fixing in vitro for chronic phase treatment effect discussion.
SMCs on a stretch chamber were heated for 15 s with stretching and fixed in a stretched formation. SMC migration is correlated with the cell respiratory activity. The amount of ATP production was measured using a WST-8 assay for respiratory activity evaluation. The intracellular expression of heat shock protein 70 was measured by an ELISA for recovery ability evaluation.
In the case of 60 °C heating, SMC respiratory activity after short-term heating/stretch-fixing decreased drastically in all stretching rates. In the case of 50 °C heating, SMC respiratory activity decreased and then increased. Alternatively, the recovery ability at 60 °C was greater than that at 50 °C.
SMCs heated at 60 °C with stretching would have high recovery ability and low respiratory activity related to SMC migration. These results may be important evidence in determining the treatment condition in PTDBA.
外周动脉狭窄治疗需要一种无支架的治疗装置。我们提出了短期加热球囊血管成形术，即光热动力球囊血管成形术（PTDBA）。尽管在猪模型中，PTDBA后的平滑肌细胞（SMC）以伸展形态固定，但这种刺激对SMC的影响尚未得到研究。血管扩张后SMC迁移可能与慢性再狭窄有关。本研究的目的是在体外对SMC进行短期加热/拉伸固定后，检测其呼吸活性和恢复能力，以探讨慢性期治疗效果。
将伸展室上的SMC在拉伸状态下加热15秒，并固定在伸展形态。SMC迁移与细胞呼吸活性相关。使用WST-8测定法测量ATP生成量以评估呼吸活性。通过ELISA测定热休克蛋白70的细胞内表达以评估恢复能力。
在60℃加热的情况下，短期加热/拉伸固定后的SMC呼吸活性在所有拉伸率下均急剧下降。在50℃加热的情况下，SMC呼吸活性先下降后上升。另外，60℃时的恢复能力大于50℃时的恢复能力。
在拉伸状态下于60℃加热的SMC具有与SMC迁移相关的高恢复能力和低呼吸活性。这些结果可能是确定PTDBA治疗条件的重要依据。
