Department of Breast Surgical Oncology, Cancer Institute and Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Eur Rev Med Pharmacol Sci. 2020 Jan;24(2):740-748. doi: 10.26355/eurrev_202001_20054.
Recently, breast cancer (BC) has become a common tumor that threatens the physical and mental health of women. Microribonucleic acids (miRNAs) have been chosen as a study object because of their roles in various cancers, including BC. Here, we mainly study the role of miR-15b in BC progression and its underlying mechanism.
Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was performed to examine the level of MiR-15b expression in 60 pairs of BC tissues and para-cancerous specimens, and the relationship between MiR-15b level and clinical features of BC patient prognosis was analyzed. MiR-15b and PAQR3 level in BC tissues and cells was tested by Western blot.
The results showed that miR-15b expression was higher and PAQR3 level was lower in BC. The identification of PAQR3 as a target of miR-15b in BC was carried out by Luciferase reporter assay and the results stated that the Luciferase activity was reduced by miR-15b mimic, indicating PAQR3 being a target of miR-15b in BC. Transwell assay was used for examining BC cell migration and invasion and found that miR-15b could promote BC cell migration and invasion, while the effect of PAQR3 was inhibition. Furthermore, PAQR3 could reverse the promotion effect of miR-15b on BC cell migratory and invasive ability. In addition, miR-15b expression was negatively correlated with PAQR3 performed by regression analysis.
Our data stated that miR-15b could facilitate BC progression via repressing tumor suppressor PAQR3, indicating that miR-15b/PAQR3 axis provided a therapeutic target for treating BC.
最近，乳腺癌（BC）已成为一种常见的肿瘤，威胁着女性的身心健康。微小 RNA（miRNAs）因其在包括 BC 在内的各种癌症中的作用而被选为研究对象。在这里，我们主要研究 miR-15b 在 BC 进展中的作用及其潜在机制。
通过定量实时聚合酶链反应（qRT-PCR）检测 60 对 BC 组织和癌旁标本中 miR-15b 的表达水平，并分析 miR-15b 水平与 BC 患者预后临床特征的关系。通过 Western blot 检测 BC 组织和细胞中的 miR-15b 和 PAQR3 水平。
结果表明，miR-15b 在 BC 中的表达较高，PAQR3 水平较低。通过荧光素酶报告基因检测证实了 PAQR3 是 miR-15b 在 BC 中的靶标，结果表明 miR-15b 模拟物降低了荧光素酶活性，表明 PAQR3 是 miR-15b 在 BC 中的靶标。通过 Transwell assay 检测 BC 细胞迁移和侵袭发现，miR-15b 可促进 BC 细胞迁移和侵袭，而 PAQR3 的作用则抑制迁移和侵袭。此外，PAQR3 可逆转 miR-15b 对 BC 细胞迁移和侵袭能力的促进作用。此外，通过回归分析发现 miR-15b 表达与 PAQR3 呈负相关。
我们的数据表明，miR-15b 可以通过抑制肿瘤抑制因子 PAQR3 促进 BC 的进展，表明 miR-15b/PAQR3 轴为治疗 BC 提供了一个治疗靶点。
