China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Int J Syst Evol Microbiol. 2020 Mar;70(3):1861-1867. doi: 10.1099/ijsem.0.003984. Epub 2020 Feb 4.
A novel strain, designated AQ6-296, was isolated from a soil sample collected in Fildes Peninsula, Antarctic. Cells were Gram-stain-negative, non-endospore-forming, non-motile, strictly aerobic and rod-shaped. Growth occurred at 4-28 °C (optimum, 20 °C) and at pH 6.0-7.0 (optimum, pH 7.0). NaCl was not obligatory for growth. Colonies were pale yellow after growth for 3 days at 20 °C on Reasoner's 2A agar. The strain was weakly positive for oxidase and the catalase test was negative. The only respiratory quinone was Q-8. The predominant cellular fatty acids were iso-C, iso-C, iso-C 3OH, summed feature 3 (comprising Cω7 and/or Cω6) and summed feature 9 (comprising iso-Cω9 and/or C10-methyl). The major polar lipids were phosphatidylethanolamine, unknown aminolipids, phosphatidylglycerol and diphosphatidylglycerol. The results of phylogenetic analysis based on 16S rRNA gene sequences (the highest similarity at 92.4 % to ) indicated that strain AQ6-296 is within the family . The DNA G+C content of the type strain was 58.6 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain AQ6-296 is considered to represent a novel genus and species in the family , for which the name gen. nov., sp. nov. is proposed. The type strain is AQ6-296 (CCTCC AB 2016313=KCTC 52744).
一株新菌株,命名为 AQ6-296,从南极菲尔德斯半岛采集的土壤样本中分离得到。细胞革兰氏染色阴性,不形成芽孢,不运动,严格需氧,呈杆状。生长在 4-28°C(最佳温度 20°C)和 pH 6.0-7.0(最佳 pH 7.0)。生长不需要 NaCl。在 20°C 培养 3 天后,在 Reasoner 2A 琼脂上,菌落呈淡黄色。该菌株氧化酶弱阳性,过氧化氢酶试验阴性。唯一的呼吸醌是 Q-8。主要的细胞脂肪酸是 iso-C、iso-C、iso-C 3OH、特征 3(包括 Cω7 和/或 Cω6)和特征 9(包括 iso-Cω9 和/或 C10-甲基)。主要的极性脂是磷脂酰乙醇胺、未知的氨基脂、磷脂酰甘油和双磷脂酰甘油。基于 16S rRNA 基因序列的系统发育分析结果(与最高相似度为 92.4%的)表明,菌株 AQ6-296 属于 科。模式菌株的 DNA G+C 含量为 58.6mol%。基于表型、化学分类和系统发育数据,菌株 AQ6-296 被认为代表了科中的一个新属和新种,建议采用新属名 ,新种名 。模式菌株是 AQ6-296(CCTCC AB 2016313=KCTC 52744)。