Andersson G L, Skottner A, Jennische E
Department of Histology, University of Göteborg, Sweden.
Acta Endocrinol (Copenh). 1988 Dec;119(4):555-60. doi: 10.1530/acta.0.1190555.
The localization of insulin-like growth factor I (IGF-I, somatomedin C) was investigated in the kidney of adult rats during normal conditions and after nephrectomy, using immunocytochemical and biochemical methods. In the normal kidney, IGF-I immunoreactivity could be demonstrated mainly in cells in the medullary collecting ducts and in those parts of the thin limb of Henle's loop located in the outer medulla. During compensatory growth all parts of the collecting ducts, including those in the cortex, showed IGF-I immunoreactivity, as did cells in the entire thin limb of Henle's loop. No IGF-I immunoreactivity could be demonstrated in the proximal or distal tubules, either in the control kidney or during compensatory growth. Biochemical measurements showed a significantly higher content of IGF-I in the inner medulla than in the cortex in the normal kidney. Uninephrectomy resulted in significantly increased IGF-I content in the cortex. It is suggested that IGF-I is produced mainly in the collecting ducts and in the thin limb of Henle's loop and exerts its effect on other parts of the nephron by paracrine mechanisms.
采用免疫细胞化学和生化方法，对成年大鼠正常状态下及肾切除术后肾脏中胰岛素样生长因子I（IGF-I，生长调节素C）的定位进行了研究。在正常肾脏中，IGF-I免疫反应性主要见于髓质集合管的细胞以及位于外髓质的亨氏袢细段的相应部位。在代偿性生长期间，集合管的所有部分，包括皮质中的集合管，均显示出IGF-I免疫反应性，亨氏袢细段的所有细胞也如此。无论是在对照肾脏还是在代偿性生长期间，近端或远端小管均未显示出IGF-I免疫反应性。生化检测表明，正常肾脏内髓质中IGF-I的含量明显高于皮质。单侧肾切除导致皮质中IGF-I含量显著增加。提示IGF-I主要在集合管和亨氏袢细段产生，并通过旁分泌机制对肾单位的其他部分发挥作用。
