School of Microbiology, University College Cork, T12 K8AF, Ireland.
APC Microbiome Ireland, University College Cork, T12 YT20, Ireland.
Future Microbiol. 2019 Dec;14:1573-1587. doi: 10.2217/fmb-2019-0153. Epub 2020 Feb 5.
To determine if bacteriocins improve antibiotic efficacy. Deferred antagonism assays identified bacteriocins with activity. Growth curves and time kill assays demonstrated bactericidal activity of antimicrobial combinations, and checkerboard assays confirmed synergy. Methicillin-resistant -infected porcine skin model determined efficacy. Subinhibitory concentrations of lacticin with penicillin or vancomycin resulted in complete growth inhibition of strains and the improved inhibitory effect was apparent after 1 h. Nisin with methicillin proved more effective against methicillin-resistant than either antimicrobial alone, revealing partial synergy and significantly reduced pathogen numbers on porcine skin after 3 h compared with minimal inhibition for either antimicrobial alone. Nisin Z and lacticin 3147 may support the use of certain antibiotics and revive ineffective antibiotics.
为了确定细菌素是否能提高抗生素的疗效。延迟拮抗试验确定了具有活性的细菌素。生长曲线和时间杀伤试验显示了抗菌组合的杀菌活性，棋盘试验证实了协同作用。耐甲氧西林感染的猪皮模型确定了疗效。乳酸链球菌素与青霉素或万古霉素的亚抑菌浓度导致菌株完全生长抑制，并且在 1 小时后抑制效果明显。与单独使用任何一种抗菌药物相比，尼生素与甲氧西林对耐甲氧西林金黄色葡萄球菌更有效，显示出部分协同作用，并且与单独使用任何一种抗菌药物的最小抑制作用相比，在 3 小时后猪皮上的病原体数量显著减少。尼生素 Z 和乳链菌肽 3147 可能支持某些抗生素的使用并恢复无效的抗生素。
