Department of Surgical Oncology, First Affiliated Hospital of China Medical University, Shenyang, China.
Technol Cancer Res Treat. 2020 Jan-Dec;19:1533033819901126. doi: 10.1177/1533033819901126.
SRY-related HMG box-12, which is associated with the prognosis of cancer, has been frequently described. However, both SRY-related HMG box-12 expression and its relationship with clinicopathological variables and patient survival have not been defined in gastric cancer. The aim of our study was to examine the prognostic value of SRY-related HMG box-12 expression in patients with gastric cancer.
In this study, we determined SRY-related HMG box-12 expression in 79 primary gastric cancer tissues and 79 matched adjacent nontumor tissues by immunohistochemistry and then calculated the survival rate using the Kaplan-Meier method. Cox proportional hazard regression model was used to analyze predictors of gastric cancer. Western blot and quantitative real-time polymerase chain reaction were used to investigate the difference in SRY-related HMG box-12 expression between normal gastric epithelial cells and gastric cancer cells at the protein level and RNA level, respectively.
SRY-related HMG box-12 was downregulated in gastric cancer tissues. Low SRY-related HMG box-12 expression was significantly associated not only with lymph node metastasis ( = .027) and TNM stage ( = .021) but also with disease-specific survival in patients with gastric cancer. Multivariate analysis demonstrated TNM stage was an independent factor predicting poor survival ( = .034).
Low SRY-related HMG box-12 expression is associated with poor clinical outcomes in gastric cancer.
与癌症预后相关的 SRY 相关 HMG 盒 12 已被频繁描述。然而，在胃癌中，SRY 相关 HMG 盒 12 的表达及其与临床病理变量和患者生存的关系尚未确定。我们的研究目的是检验 SRY 相关 HMG 盒 12 在胃癌患者中的预后价值。
在这项研究中，我们通过免疫组织化学法测定了 79 例原发性胃癌组织和 79 例配对的非肿瘤组织中的 SRY 相关 HMG 盒 12 表达，并通过 Kaplan-Meier 法计算生存率。Cox 比例风险回归模型用于分析胃癌的预测因素。Western blot 和定量实时聚合酶链反应分别用于在蛋白水平和 RNA 水平上研究正常胃上皮细胞和胃癌细胞之间 SRY 相关 HMG 盒 12 表达的差异。
SRY 相关 HMG 盒 12 在胃癌组织中下调。低 SRY 相关 HMG 盒 12 表达不仅与淋巴结转移（ =.027）和 TNM 分期（ =.021）显著相关，而且与胃癌患者的疾病特异性生存显著相关。多因素分析表明 TNM 分期是预测不良生存的独立因素（ =.034）。
低 SRY 相关 HMG 盒 12 表达与胃癌的不良临床结局相关。
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