肝片吸虫和枝双腔吸虫：苹果酸脱氢酶和苹果酸酶的同工酶模式
Fasciola hepatica and Dicrocoelium dendriticum: isoenzyme patterns of malate dehydrogenase and malic enzyme.
作者信息
Leon-Ortega P, Monteoliva M, Sanchez-Moreno M
机构信息
Sección de Bioquímica del Instituto Lopez-Neyra de Granada, C.S.I.C., Spain.
出版信息
Angew Parasitol. 1988 Sep;29(3):173-7.
The MDH isoenzymes and ME isoenzymes (EC.1.1.1.39) in two helminth parasites, F. hepatica and D. dendriticum, obtained from two host species (Capra hircus and Ovis aries), were studied by electrophoresis on polyacrylamide gels. F. hepatica MDH showed three isoenzymatic bands; D. dendriticum MDH, only two. No difference was noted between the enzyme profiles, or the densitometric scans in samples from specimens from different host species. F. hepatica ME presented three bands, that of D. dendriticum, only two. Only in F. hepatica were differences observed between the enzyme profiles of specimens obtained from the different host species.
采用聚丙烯酰胺凝胶电泳法，对从两种宿主物种（山羊和绵羊）体内获取的两种蠕虫寄生虫——肝片吸虫和枝双腔吸虫中的苹果酸脱氢酶（MDH）同工酶和苹果酸酶（ME，EC.1.1.1.39）进行了研究。肝片吸虫的MDH显示出三条同工酶带；枝双腔吸虫的MDH仅显示出两条。不同宿主物种样本的酶谱或光密度扫描结果未发现差异。肝片吸虫的ME呈现出三条带，枝双腔吸虫的ME仅呈现出两条。仅在肝片吸虫中，观察到了来自不同宿主物种的样本在酶谱上的差异。
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