Batool Azra, Miana Ghulam Abbas, Muddassir Muhammad, Khan Mohsin Abbas, Zafar Saba
Department of Pharmaceutical Chemistry, Riphah Institute of Pharmaceutical Sciences, Riphah International University, Islamabad, Pakistan.
Department of Pharmaceutical Chemistry, Riphah Institute of Pharmaceutical Sciences, Riphah International University, Islamabad, Pakistan/Amson Vaccines & Pharma Quality Control Laboratory, Islamabad, Pakistan.
Pak J Pharm Sci. 2019 Nov;32(6(Supplementary)):2771-2777.
Medicinal plants are proven to reveal vast promising potential providing novel drug candidates to combat health-related problems. The aim of current study is to discover new drug compounds with anti-anticancer, antioxidant, antibacterial and antifungal potential, to serve the purpose Saussurea heteromalla (Family: Asteraceae) indigenous to Pakistan was screened for the in vitro cytotoxicity against HeLa cells (Human cervical cancer cell line) compared to the NIH / 3T3 cells (mouse normal fibroblast cells) by performing the MTT colorimetric assay and antifungal, antibacterial and antioxidant potential by adopting standard protocols. S. heteromalla crude methanolic extract (CME) demonstrated strong cytotoxic potential against HeLa cells at 200μg/mL; (77.28 ±1.53% kill; IC: 62.13μg/mL) compared to standard doxorubicin (95.90% kill; IC50: 0.2μg/mL). Inhibitory Zone of the extract at concentrations (30, 60, 90μg/mL) against Bacillus subtilis, Serratia marecescens, Staphylococcus aureus, Micrococcus luteus, Pseudomonas aeruginosa, Strptotropomonas maltophilia, Escherichia coli, Salmonella typhi, and Saccharomyces cerevisiae, Candida albicans, Candida glabrata was measured. Manifestation of intensified results against Gram-negative Serratia marecescens qualifies the S. heteromalla extract as a considerable source of narrow spectrum antibiotic. However, antifungal activity against C. albicans was found to be logical. Antioxidant potential was determined through DPPH assay which declared no notable antioxidant effects. To the best of our knowledge this is first research and report on above mentioned biological studies of S. heteromalla.
药用植物已被证明具有巨大的潜在前景，可为解决健康相关问题提供新的候选药物。本研究的目的是发现具有抗癌、抗氧化、抗菌和抗真菌潜力的新药物化合物。为此，对原产于巴基斯坦的异叶风毛菊（菊科）进行了研究，通过MTT比色法检测其对HeLa细胞（人宫颈癌细胞系）的体外细胞毒性，并与NIH / 3T3细胞（小鼠正常成纤维细胞）进行比较，同时采用标准方案检测其抗真菌、抗菌和抗氧化潜力。异叶风毛菊粗甲醇提取物（CME）在200μg/mL时对HeLa细胞显示出较强的细胞毒性；（杀伤率为77.28±1.53%；IC：62.13μg/mL），而标准阿霉素的杀伤率为95.90%；IC50为0.2μg/mL。测定了该提取物在浓度（30、60、90μg/mL）下对枯草芽孢杆菌、粘质沙雷氏菌、金黄色葡萄球菌、藤黄微球菌、铜绿假单胞菌、嗜麦芽窄食单胞菌、大肠杆菌、伤寒沙门氏菌以及酿酒酵母、白色念珠菌、光滑念珠菌的抑菌圈。对革兰氏阴性菌粘质沙雷氏菌的增强结果表明，异叶风毛菊提取物是窄谱抗生素的重要来源。然而，对白色念珠菌的抗真菌活性是合理的。通过DPPH测定法确定了抗氧化潜力，结果表明没有显著的抗氧化作用。据我们所知，这是关于异叶风毛菊上述生物学研究的首次研究和报告。
