Bilal Shahrukh, Anam Sidra, Mahmood Tauqeer, Abdullah Rana Muhammad, Nisar Sajid, Kalsoom Furkhanda, Luqman Muhammad, Anjum Faisal Rasheed
Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan.
Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan/Poultry Research Institute, Rawalpindi.
Pak J Pharm Sci. 2019 Nov;32(6(Supplementary)):2887-2891.
Urinary tract infections (UTIs) are among the most common bacterial infections acquired from hospitals and community. Pseudomonas and Proteus species are the common cause of these UTIs. Generally, UTIs are self-limiting but have potential to re-occur. Extensive treatment therapy with antibiotics lead to the development of resistance in uropathogens. The development of antibiotic resistance is leading to the failure of currently available antibiotic based therapies thus making the situation worse. The objective of the present study was to access antimicrobial sensitivity and to characterize antibiotic resistant genes of Proteus vulgaris (P. vulgaris) isolated from patients suffering with UTIs. A total of 150 urine samples were collected and cultured on MacConkey agar medium followed by isolation and identification on blood agar medium. Biochemical characterization of all presumptive Proteus isolates was done using Remel Rap ID one kit. Antibiotic sensitivity for P. vulgaris isolates was performed by disc diffusion method. Presence of blaTEM and qnr antibiotic resistant genes was determined by PCR. The results showed that the overall prevalence of P. vulgaris in clinical samples was 11.3%. It showed maximum resistance (94%) to three antibiotics i.e. ampicillin, tigecycline and chloramphenicol, while least resistance was observed against imipenem (12%). Statistical analysis depicted that imipenem had a significantly larger zone of inhibition (P=.01), while ampicillin had significantly smaller zone of inhibition (P=.0004) followed by chloramphenicol (p-value = 0.002). Imipenem should be considered as an effective antibiotic to treat urinary tract infections associated with P. vulgaris. Both blaTEM and qnr genes were found to be involved in conferring resistance to β-lactam and quinolones antibiotics.
尿路感染（UTIs）是医院和社区获得性最常见的细菌感染之一。假单胞菌属和变形杆菌属是这些尿路感染的常见病因。一般来说，尿路感染是自限性的，但有复发的可能。使用抗生素进行广泛的治疗会导致尿路病原体产生耐药性。抗生素耐药性的发展导致目前基于抗生素的治疗方法失效，从而使情况变得更糟。本研究的目的是评估普通变形杆菌（P. vulgaris）的抗菌敏感性，并对从患有尿路感染的患者中分离出的该菌的抗生素耐药基因进行表征。总共收集了150份尿液样本，在麦康凯琼脂培养基上进行培养，随后在血琼脂培养基上进行分离和鉴定。使用Remel Rap ID one试剂盒对所有疑似变形杆菌分离株进行生化鉴定。通过纸片扩散法对普通变形杆菌分离株进行抗生素敏感性检测。通过聚合酶链反应（PCR）确定blaTEM和qnr抗生素耐药基因的存在。结果显示，临床样本中普通变形杆菌的总体患病率为11.3%。它对三种抗生素即氨苄西林、替加环素和氯霉素表现出最高耐药性（94%），而对亚胺培南的耐药性最低（12%）。统计分析表明，亚胺培南有显著更大的抑菌圈（P = 0.01），而氨苄西林有显著更小的抑菌圈（P = 0.0004），其次是氯霉素（P值 = 0.002）。亚胺培南应被视为治疗与普通变形杆菌相关的尿路感染的有效抗生素。发现blaTEM和qnr基因均参与赋予对β-内酰胺类和喹诺酮类抗生素的耐药性。
