用于体外转录起始的维生素-鸟苷一磷酸缀合物。
Vitamin-guanosine monophosphate conjugates for in vitro transcription priming.
机构信息
KU Leuven, Rega Institute for Medical Research, Medicinal Chemistry, Herestraat 49-Box 1041, 3000 Leuven, Belgium.
Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ Evry, Université Paris-Saclay, 2 Rue Gaston Crémieux, 91057 Evry, France.
出版信息
Chem Commun (Camb). 2020 Mar 3;56(18):2787-2790. doi: 10.1039/c9cc09427j.
Expanding the catalytic repertoire of ribozymes to include vitamin synthesis requires efficient labelling of RNA with the substrate of interest, prior to in vitro selection. For this purpose, we rationally designed and synthesized six GMP-conjugates carrying a synthetic pre-thiamine or biotin precursor and investigated their transcription incorporation properties by T7 RNA polymerase.
为了在体外选择之前有效地对 RNA 进行感兴趣的底物标记，需要将核酶的催化谱扩展到包括维生素合成。为此，我们合理设计和合成了六个带有合成前硫胺素或生物素前体的 GMP 缀合物，并通过 T7 RNA 聚合酶研究了它们的转录掺入特性。
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