Section of Molecular Craniofacial Embryology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Section of Oral Implantology and Regenerative Dental Medicine, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Odontology. 2020 Jul;108(3):386-395. doi: 10.1007/s10266-020-00489-w. Epub 2020 Feb 5.
There exists a close connection between changes occurring in the teeth and those occurring in the jaw during the evolutionary process. In mammals, the roots of teeth are supported, along with periodontal ligaments and alveolar bones by a unique structure termed the gomphosis. In the present study, we performed combined in silico analysis using the information obtained from various DNA microarrays and identified 19 putative tooth root formation-related genes. Furthermore, quantitative PCR was performed on the candidate genes, Chd3 was confirmed as having sufficient expression levels in the early stage of tooth root formation and increased gene expression toward the middle stage. A high degree of Chd3 gene expression was observed in secretory ameloblasts and Hertwig's epithelial root sheath (HERS), but low expression was observed in developing odontoblasts and stellate reticulum. The CHD3 foci were observed in the nucleus of the HERS01a cells. In addition, knockdown experiments using SiChd3 suggested the involvement of Chd3 in the suppression of DNA synthesis. These results suggested that Chd3 plays a role in DNA synthesis in HERS cells for promoting tooth root development.
在进化过程中，牙齿和颌骨的变化之间存在密切的联系。在哺乳动物中，牙齿的根部与牙周韧带和牙槽骨一起被一种称为关节的独特结构所支撑。在本研究中，我们使用从各种 DNA 微阵列获得的信息进行了联合的计算机分析，并鉴定了 19 个可能与牙齿根部形成相关的基因。此外，对候选基因进行了定量 PCR 分析，结果证实 Chd3 在牙齿根部形成的早期阶段具有足够的表达水平，并在中期阶段增加了基因表达。Chd3 基因在分泌型成釉细胞和 Hertwig 上皮根鞘 (HERS) 中具有高度表达，但在发育中的成牙本质细胞和成星形网状细胞中表达水平较低。CHD3 焦点存在于 HERS01a 细胞的核中。此外，使用 SiChd3 进行的敲低实验表明 Chd3 参与抑制 DNA 合成。这些结果表明 Chd3 在促进牙齿根部发育的 HERS 细胞的 DNA 合成中发挥作用。
