Comparative Ophthalmology Lab (LABOCO), Curitiba, Brazil.
Veterinary Teaching Hospital - UFPR, Curitiba, Brazil.
Vet Ophthalmol. 2020 May;23(3):497-505. doi: 10.1111/vop.12746. Epub 2020 Feb 5.
To evaluate in vitro the antibacterial effects of fluorescein, rose bengal, and lissamine green topical ophthalmic dyes against selected Gram-positive and Gram-negative bacteria, and to evaluate whether preserved or preservative-free fluorescein solutions are able to inhibit or potentiate bacterial growth.
Susceptibility testing was performed using the Kirby-Bauer disk diffusion method plated with clinical ocular isolates of Staphylococcus aureus, Staphylococcus pseudintermedius, Streptococcus spp., Escherichia coli, and Pseudomonas aeruginosa. Bacterial growth inhibition was evaluated 24 hours following the addition of commercially available fluorescein, rose bengal, and lissamine green sterile strips. Antimicrobial effectiveness testing was performed by inoculation of compounded 1% dye solutions, both with and without preservatives (fluorescein and lissamine contained thiomersal, and rose bengal contained nipagin and nepazol), with the five previously mentioned bacteria. Growth was evaluated at days 7, 14, and 28.
All dyes showed antibacterial activity against Gram-positive organisms. Preservative-free compounded 1% fluorescein solution inhibited growth of Gram-positive organisms but not of Gram-negative organisms. Preservative-free rose bengal and lissamine green inhibited growth of both types of organisms.
Preferably, ocular surface samples for antimicrobial culture should be taken prior to the administration of topical dyes, due to their potential antibacterial activity, particularly if undiluted strips are applied directly or commercial fluorescein solutions are used and not immediately rinsed. Ophthalmic dye solutions containing preservative are safe from bacterial growth for up to 28 days if properly handled and stored. The use of preservative-free fluorescein solutions should be avoided and preservative-free rose bengal and lissamine green should be handled carefully.
评估荧光素、孟加拉玫瑰红和丽丝胺绿三种眼部外用染色剂对选定的革兰氏阳性和革兰氏阴性细菌的体外抗菌效果，并评估是否保存或不保存荧光素溶液能够抑制或增强细菌生长。
使用 Kirby-Bauer 圆盘扩散法对临床眼部分离的金黄色葡萄球菌、中间葡萄球菌、链球菌属、大肠杆菌和铜绿假单胞菌进行药敏试验。添加市售荧光素、孟加拉玫瑰红和丽丝胺绿无菌条 24 小时后，评估细菌生长抑制情况。通过接种有和没有防腐剂（荧光素和丽丝胺含有硫柳汞，孟加拉玫瑰红含有尼泊金和尼泊唑）的 1%复合染色剂溶液进行抗菌有效性测试，对上述五种细菌进行测试。在第 7、14 和 28 天评估生长情况。
所有染料均对革兰氏阳性菌具有抗菌活性。不含防腐剂的复合 1%荧光素溶液抑制革兰氏阳性菌的生长，但不抑制革兰氏阴性菌的生长。不含防腐剂的孟加拉玫瑰红和丽丝胺绿抑制了两种类型的细菌的生长。
由于这些染料具有潜在的抗菌活性，特别是如果直接应用未稀释的条带或使用未立即冲洗的商业荧光素溶液，则在进行局部染色剂治疗之前，最好在进行眼部表面样本的抗菌培养，因为这些染料具有潜在的抗菌活性。如果正确处理和储存，含有防腐剂的眼用染色剂溶液在 28 天内可安全防止细菌生长。应避免使用不含防腐剂的荧光素溶液，并应小心使用不含防腐剂的孟加拉玫瑰红和丽丝胺绿。
