Pang Xing-Chen, Li Hong-Jiao, Wang Yi, Guan Feng, Li Xiang
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu Province，China.
Joint International Research Laboratory of Glycobiology and Medicinal Chemistry, College of Life Science, Northwest University, Xi'an 710069, Shaanxi Province，China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2020 Feb;28(1):283-289. doi: 10.19746/j.cnki.issn.1009-2137.2020.01.047.
To investigate the effect of bone marrow stromal cell glycosyltransferase B4GALT1 expression on hematopoietic cell proliferation and its upstream regulation mechanism.
B4GALT1 was overexpressed in human bone marrow stromal cell line HS5, which was then co-cultured with acute myeloid leukemia cell line KG1a. And its effect on hematopoietic cell proliferation was detected by flow cytometry. Dual luciferase reporter assay, real-time PCR and Western blot were used to predict and validate upstream transcription factors that regulate stromal cell B4GALT1 expression.
Overexpression of B4GALT1 in HS5 significantly promoted the proliferation of KG1a in the co-culture system. B4GALT1 expression in stromal cells positively correlated with upstream c-Jun expression, which was verified by JNK/c-Jun inhibitors.
The differential expression of glycosyltransferases and their corresponding glycosylation in the hematopoietic microenvironment play an important role.
探讨骨髓基质细胞糖基转移酶B4GALT1表达对造血细胞增殖的影响及其上游调控机制。
在人骨髓基质细胞系HS5中过表达B4GALT1，然后将其与急性髓系白血病细胞系KG1a共培养。通过流式细胞术检测其对造血细胞增殖的影响。采用双荧光素酶报告基因检测、实时定量PCR和蛋白质免疫印迹法预测并验证调控基质细胞B4GALT1表达的上游转录因子。
HS5中B4GALT1的过表达显著促进了共培养体系中KG1a的增殖。基质细胞中B4GALT1的表达与上游c-Jun的表达呈正相关，JNK/c-Jun抑制剂验证了这一点。
造血微环境中糖基转移酶及其相应糖基化的差异表达起重要作用。
