EBSI Team, Interdisciplinary Chemistry: Synthesis, Analysis, Modelling (CEISAM), University of Nantes - CNRS UMR 6230, 2 rue de la Houssinière, BP 92208, F-44322 Nantes Cedex 3, France; Laboratory of Metrology and Isotopic Fractionation, Research Unit: Technologies et Valorisation Agroalimentaire (TVA), Faculty of Science, Saint Joseph University of Beirut, P.O. Box 17-5208 Mar Mikhael, Beirut 1104 2020, Lebanon.
Laboratory of Metrology and Isotopic Fractionation, Research Unit: Technologies et Valorisation Agroalimentaire (TVA), Faculty of Science, Saint Joseph University of Beirut, P.O. Box 17-5208 Mar Mikhael, Beirut 1104 2020, Lebanon.
Food Chem. 2020 Jun 15;315:126325. doi: 10.1016/j.foodchem.2020.126325. Epub 2020 Jan 30.
In previous works, we developed a C NMR method for analyzing triacylglycerols in olive oil using an adiabatic refocused INEPT sequence. This allowed spectral acquisition to be done in only 8 min with sufficient precision for isotopic measurements. In the present study, we made use of the same methodology to investigate the potential of triacylglycerols as source of biomarkers in animal origin matrices. To this end, egg yolk was taken as a model matrix. We called our profiling approach metabisotopomics since it was simultaneously metabolomic and isotopic profiling. Beside its ability to quantitate several fatty acids, metabisotopomics of triacylglycerols in egg yolk allowed the multivariate classification of samples according to the hen breed, to the farming system and origin. Achieved results confirmed our presumption that C metabisotopomics of triacylglycerols from animal sources is a powerful tool for metabolic studies as well as for food authentication processes.
在之前的工作中，我们开发了一种使用绝热重聚焦 INEPT 序列分析橄榄油中三酰基甘油的 13C NMR 方法。这使得仅需 8 分钟即可完成光谱采集，并且具有足够的同位素测量精度。在本研究中，我们利用相同的方法学来研究三酰基甘油作为动物源基质中生物标志物来源的潜力。为此，蛋黄被用作模型基质。我们将我们的分析方法称为代谢同位素组学，因为它同时进行代谢组学和同位素分析。除了能够定量几种脂肪酸之外，蛋黄三酰基甘油的代谢同位素组学还能够根据母鸡品种、养殖系统和来源对样品进行多元分类。所得结果证实了我们的假设，即动物源三酰基甘油的 C 代谢同位素组学是代谢研究以及食品认证过程的有力工具。
