Hilsted L, Bardram L, Rehfeld J F
University Department of Clinical Chemistry, Rigshospitalet, Copenhagen, Denmark.
Biochem J. 1988 Oct 15;255(2):397-402. doi: 10.1042/bj2550397.
The post-translational maturation of antral progastrin was studied in the developing rat. While N-terminal proteolysis remained unchanged and tyrosine O-sulphation varied only slightly during ontogenesis, major changes were observed in the degree of alpha-carboxyamidation. In the third week of life the immediate precursor of amidated gastrin, glycine-extended gastrin, accumulated, and at weaning (day 21) the concentrations exceeded those of amidated gastrin. Our results confirm that weaning is accompanied by an increased synthesis of gastrin and imply that alpha-carboxyamidation is the rate-limiting step during the biosynthetic maturation of gastrin.
在发育中的大鼠中研究了胃窦前胃泌素的翻译后成熟过程。在个体发育过程中，N端蛋白水解保持不变，酪氨酸O-硫酸化仅有轻微变化，但α-羧基酰胺化程度出现了显著变化。在出生后的第三周，酰胺化胃泌素的直接前体——甘氨酸延伸胃泌素开始积累，到断奶时（第21天），其浓度超过了酰胺化胃泌素。我们的结果证实，断奶伴随着胃泌素合成的增加，并表明α-羧基酰胺化是胃泌素生物合成成熟过程中的限速步骤。
