Conrad K, Karsten U, Frank K H, Pasternak G
Central Institute of Molecular Biology, Academy of Sciences of the GDR, Berlin-Buch.
Biomed Biochim Acta. 1988;47(7):697-703.
Cytoskeletal extracts of HeLa and MCF-7 cells were separated by SDS polyacrylamide gel electrophoresis, electrotransferred to nitrocellulose paper and exposed to five of our monoclonal antibodies (mabs) against structures of the cytoplasm as demonstrated in indirect immunofluorescence. Three of them reacted with cytokeratins according to histological evidence. All mabs tested in this study were reactive in immunoblotting. The broad-range anti-cytokeratin antibody A45-B/B3 was shown to react with cytokeratin polypeptides 8 and 18 and possibly 7, but not with cytokeratin 17.A53-B/A2 bound only to cytokeratin 19.Mab A 51-B/H4 showed a reaction with cytokeratin 18. Two mabs not yet described in detail (A45-C/H1 and A45-C/H9) reacted with a single band of 25 kD and may detect a cytoskeleton-associated protein.
将HeLa细胞和MCF - 7细胞的细胞骨架提取物通过SDS聚丙烯酰胺凝胶电泳进行分离，电转移至硝酸纤维素膜上，并与我们针对细胞质结构的五种单克隆抗体（mabs）进行反应，如间接免疫荧光所示。根据组织学证据，其中三种抗体与细胞角蛋白发生反应。在本研究中测试的所有单克隆抗体在免疫印迹中均有反应。广谱抗细胞角蛋白抗体A45 - B/B3显示与细胞角蛋白多肽8和18以及可能的7发生反应，但不与细胞角蛋白17发生反应。A53 - B/A2仅与细胞角蛋白19结合。单克隆抗体A 51 - B/H4与细胞角蛋白18发生反应。两种尚未详细描述的单克隆抗体（A45 - C/H1和A45 - C/H9）与一条25 kD的条带发生反应，可能检测到一种细胞骨架相关蛋白。
