血小板活化因子（PAF）对豚鼠骨髓细胞DNA合成的增强作用。

Augmentation of DNA synthesis in guinea pig bone marrow cells by platelet-activating factor (PAF).

作者信息

Kato T, Kudo I, Hayashi H, Onozaki K, Inoue K

机构信息

Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.

出版信息

Biochem Biophys Res Commun. 1988 Dec 15;157(2):563-8. doi: 10.1016/s0006-291x(88)80286-9.

DOI:10.1016/s0006-291x(88)80286-9

PMID:3202869

Abstract

When guinea pig bone marrow cells were incubated in the presence of 10(-7) to 10(-5) M platelet-activating factor (PAF) for 24 to 72 hours, [3H]thymidine incorporation of cells was time-dependently augmented. The enantiomer of PAF and lysoPAF, a major metabolite of PAF, did not show significant enhancement. A non-metabolizable potent PAF agonist, 1-O-octadecyl-2-O-(N,N-dimethylcarbamoyl)-sn-glycero-3-phospholine, enhanced the [3H]thymidine incorporation at 10(-10) to 10(-8) M. This augmentation of DNA synthesis in bone marrow cells was abolished by specific PAF antagonists, CV-6209 or FR-900452. When the conditioned medium of PAF-stimulated bone marrow cells was added to another culture of bone marrow cells, the augmentation of DNA synthesis was also observed. These results suggest that PAF may affect the proliferation of one or some classes of guinea pig bone marrow cells through release of soluble factor(s).

摘要

当豚鼠骨髓细胞在10⁻⁷至10⁻⁵M血小板活化因子（PAF）存在的情况下孵育24至72小时时，细胞的[³H]胸苷掺入呈时间依赖性增加。PAF的对映体以及PAF的主要代谢产物溶血PAF均未显示出显著增强作用。一种不可代谢的强效PAF激动剂，1-O-十八烷基-2-O-（N，N-二甲基氨基甲酰基）-sn-甘油-3-磷酸胆碱，在10⁻¹⁰至10⁻⁸M时增强了[³H]胸苷掺入。骨髓细胞中DNA合成的这种增加被特异性PAF拮抗剂CV-6209或FR-900452消除。当将PAF刺激的骨髓细胞的条件培养基添加到另一骨髓细胞培养物中时，也观察到了DNA合成的增加。这些结果表明，PAF可能通过释放可溶性因子来影响一类或某些类别的豚鼠骨髓细胞的增殖。