Bazhenov Mikhail, Chernook Anastasiya, Kroupin Pavel, Karlov Gennady, Divashuk Mikhail
Laboratory of Applied Genomics and Crop Breeding, All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya street, 42, 127550 Moscow, Russia.
Kurchatov Genomics Center of All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya str. 42, 127550 Moscow, Russia.
Plants (Basel). 2020 Feb 3;9(2):186. doi: 10.3390/plants9020186.
The gene, first studied in rice, encodes a protein that acts as a repressor of the physiological response of plants to strigolactones-substances that regulate the activity of axillary buds, stem growth, branching of roots and other physiological processes. In this work, we isolated and sequenced the homolog of the gene in several accessions of the wild grass of different geographical origins, resulting in the discovery of large allelic variety. A molecular marker was also created that allows us to differentiate the gene from common wheat genes. Using this marker and monosomic addition, substitution and translocation wheat lines carrying the known chromosomes, the gene was localized on the long arm of the 5V chromosome.
该基因最初是在水稻中研究的,它编码一种蛋白质,该蛋白质作为植物对独脚金内酯生理反应的抑制因子,独脚金内酯是调节腋芽活性、茎生长、根分支及其他生理过程的物质。在这项研究中,我们分离并测序了不同地理来源的野生草几个品种中该基因的同源基因,从而发现了大量的等位基因变异。我们还创建了一个分子标记,使我们能够将该基因与普通小麦基因区分开来。利用这个标记以及携带已知染色体的单体附加、替换和易位小麦品系,该基因被定位在5V染色体的长臂上。