Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou, 510632, China; International Cooperative Laboratory of Traditional Chinese Medicine Modernization, Innovative Drug Development of Chinese Ministry of Education (MOE), School of Pharmacy, Jinan University, 601 Huangpu Avenue West, Guangzhou, 510632, China; Department of Pharmacy, Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou, 510632, China.
Department of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
Anal Chim Acta. 2020 Mar 8;1101:222-229. doi: 10.1016/j.aca.2019.12.016. Epub 2019 Dec 9.
In this study, a series of zwitterionic phosphorylcholine functionalized monolithic columns were fabricated via the thermally initiated co-polymerization of 2-methacryloyloxyethyl phosphorylcholine (MPC) and different hydrophilic crosslinkers, including 1,4-bis(acryloyl)piperazine (PDA), N,N'-methylenebisacrylamide (MBA) and 2-(methacryloyloxy)ethyl-[N-(2-methacryloyloxy)ethyl]phosphorylcholine (MMPC). The physicochemical and chromatographic properties of these MPC functionalized monoliths, including column morphology, pore size distribution, permeability, column efficiency, retention mechanism and ζ-potential analysis, were systematically compared. Furthermore, the influence of the crosslinker on the chromatographic performance of these MPC functionalized monoliths was evaluated. The chromatographic results indicate that the polarity of MPC functionalized monoliths may be related to the polarity of the crosslinker, which further affects the column selectivity and efficiency. A particularly high column efficiency (88,000 plates/m) was obtained on the novel poly(MPC-co-MMPC) monolith at optimum linear velocity using thiourea as test analyte. Compared to the poly(MPC-co-MBA) and poly(MPC-co-PDA) monoliths, the poly(MPC-co-MMPC) monolith exhibited higher separation selectivity for polar analytes, including nucleobases, nucleosides and benzoic acid derivatives. Moreover, 24 N-glycopeptides could be detected after enrichment with the poly(MPC-co-MMPC) versus 19 and 10 N-glycopeptides with the poly(MPC-co-MBA) and poly(MPC-co-PDA) monoliths, and no N-glycopeptide without enrichment. Therefore, MMPC has a great potential as a new and alternative hydrophilic crosslinker for the development of zwitterionic polymeric monoliths.
在这项研究中，通过热引发 2-甲基丙烯酰氧基乙基磷酸胆碱（MPC）与不同亲水性交联剂（包括 1,4-双（丙烯酰基）哌嗪（PDA）、N,N'-亚甲基双丙烯酰胺（MBA）和 2-（甲基丙烯酰氧基）乙基-[N-（2-甲基丙烯酰氧基）乙基]磷酸胆碱（MMPC）的共聚反应，制备了一系列两性离子磷酸胆碱功能化整体柱。这些 MPC 功能化整体柱的物理化学和色谱性能，包括柱形态、孔径分布、渗透性、柱效、保留机制和 ζ-电位分析，进行了系统比较。此外，还评估了交联剂对这些 MPC 功能化整体柱色谱性能的影响。色谱结果表明，MPC 功能化整体柱的极性可能与交联剂的极性有关，进而影响柱的选择性和效率。在最佳线性流速下，使用硫脲作为测试分析物，在新型聚（MPC-co-MMPC）整体柱上获得了特别高的柱效（88,000 板/m）。与聚（MPC-co-MBA）和聚（MPC-co-PDA）整体柱相比，聚（MPC-co-MMPC）整体柱对极性分析物（包括碱基、核苷和苯甲酸衍生物）表现出更高的分离选择性。此外，用聚（MPC-co-MMPC）可以富集到 24 个 N-糖肽，而用聚（MPC-co-MBA）和聚（MPC-co-PDA）整体柱只能富集到 19 个和 10 个 N-糖肽，并且没有未经富集的 N-糖肽。因此，MMPC 作为两性离子聚合物整体柱开发的新型替代亲水性交联剂具有很大的潜力。
