Yang Wengang, Wen Dezhong, Chen Shaoxi, Xue Song, Gu Jianmin, Dan Jianggui, Zheng Hui
Department of Cardiovascular Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
Department of Cardiovascular Surgery, Zhe Jiang Hospital, Hangzhou 310030, China.
J Thorac Dis. 2019 Dec;11(12):4960-4965. doi: 10.21037/jtd.2019.12.32.
To detect the expression of Nesprin-1 in aortic dissection (AD) in patients and to investigate the role of Nesprin-1 in the pathogenesis of AD in a mouse model.
Blood and tissue specimens from AD patients were collected. The expression of Nesprin-1 in tissues from AD patients and non-AD patients with heart disease was studied by western blotting and quantitative real-time polymerase chain reaction (qRT-PCR). In addition, the expression and distribution of Nesprin-1 in AD and sham mice were compared in an induced AD mouse model, and detected by immunohistochemistry and qRT-PCR.
Immunoblotting and qRT-PCR both showed that the expression of Nesprin-1 was significantly higher in AD versus control patients. An animal model of AD was established by continuous injection of Ang II into ApoE mice. The expression of Nesprin-1 in aortic tissue of AD mice was higher than that of sham-operated mice as determined by immunohistochemistry. qRT-PCR showed that Nesprin-1 gene expression in aorta of AD mice was higher than that of sham-operated mice.
An increased expression of Nesprin-1 was associated with AD, and hence Nesprin-1 may be involved in the pathogenesis of ADs. Preliminary findings suggest that Nesprin-1 may be a therapeutic target for the treatment of AD.
检测Nesprin-1在主动脉夹层（AD）患者中的表达，并在小鼠模型中研究Nesprin-1在AD发病机制中的作用。
收集AD患者的血液和组织标本。采用蛋白质免疫印迹法和定量实时聚合酶链反应（qRT-PCR）研究Nesprin-1在AD患者及非AD心脏病患者组织中的表达。此外，在诱导性AD小鼠模型中比较Nesprin-1在AD小鼠和假手术小鼠中的表达及分布，并通过免疫组织化学和qRT-PCR进行检测。
免疫印迹法和qRT-PCR均显示，与对照组患者相比，AD患者中Nesprin-1的表达显著更高。通过向载脂蛋白E（ApoE）小鼠连续注射血管紧张素II（Ang II）建立AD动物模型。免疫组织化学检测显示，AD小鼠主动脉组织中Nesprin-1的表达高于假手术小鼠。qRT-PCR显示，AD小鼠主动脉中Nesprin-1基因表达高于假手术小鼠。
Nesprin-1表达增加与AD相关，因此Nesprin-1可能参与AD的发病机制。初步研究结果表明，Nesprin-1可能是治疗AD的一个治疗靶点。
