Gutiérrez-Aguirre Martha A, Cervantes-Martínez Adrián, Elías-Gutiérrez Manuel, Lugo-Vázquez Alfonso
Departamento de Ciencias y Humanidades, Universidad de Quintana Roo, Cozumel, Quintana Roo, Mexico.
Departamento de Sistemática y Ecología Acuática, El Colegio de la Frontera Sur, Chetumal, Quintana Roo, Mexico.
PeerJ. 2020 Jan 29;8:e8416. doi: 10.7717/peerj.8416. eCollection 2020.
In Mexico, species of four families of free-living calanoid copepods have been recorded as inhabitants of several freshwater systems. These families are Centropagidae, Temoridae, Pseudodiaptomidae and Diaptomidae. The genera and are the most speciose diaptomid genera in Mexico, and they inhabit natural and artificial lakes, ephemeral ponds, springs, and caverns. is considered as an endemic Nearctic genus, whereas is a widely distributed Neotropical genus in the southern USA, Mexico, the Caribbean Islands and Central America. Based on new and recent evidence, diversity has been underestimated: six species of the genus were known before 2000. In this work three new species have been described from different regions of Mexico by using integrative taxonomy. We also gave amended diagnosis of Bowman (1986) and s. str. (Kiefer, 1938).
In this work, the taxonomic status of the species was clarified using modern, integrative method based on the COI gene as a DNA marker, plus micro-structural analysis (based on SEM and ligth microscopy).
Three new species of were described based on genetic and morphological analyses: sp. n., sp. n. and sp. n. Also amended description of , morphological variation of s. str., and a comparison of them with all known sequences within the genus are provided. These new findings show that in differences in several cuticular microstructures of several appendages (such as the antennules, the fifth legs, or the urosomites of these copepods) agree with the interspecific genetic divergence >3% observed in sequences of the COI gene, and the integration of this information is a powerful tool in species delineation.
在墨西哥，已记录到四个自由生活的哲水蚤科物种是几个淡水系统的栖息者。这些科是长腹水蚤科、温剑水蚤科、伪镖水蚤科和镖水蚤科。属和属是墨西哥镖水蚤科中物种最丰富的属，它们栖息于天然和人工湖泊、临时池塘、泉水和洞穴中。属被认为是近北极地区的特有属，而属是一个广泛分布于美国南部、墨西哥、加勒比群岛和中美洲的新热带属。基于新的和最近的证据，属的多样性被低估了：2000年之前已知该属有6个物种。在这项工作中，通过综合分类学从墨西哥不同地区描述了3个属的新物种。我们还对鲍曼（1986年）的属和狭义的属（基弗，1938年）进行了修订诊断。
在这项工作中，使用基于细胞色素氧化酶亚基I（COI）基因作为DNA标记的现代综合方法，加上微观结构分析（基于扫描电子显微镜和光学显微镜），阐明了物种的分类地位。
基于遗传和形态分析描述了3个属的新物种：新种、新种和新种。还提供了属的修订描述、狭义属的形态变异，以及它们与该属内所有已知序列的比较。这些新发现表明，在属中，几种附肢（如这些桡足类的触角、第五对腿或尾节）的几个表皮微观结构差异与COI基因序列中观察到的种间遗传差异>3%一致，并且整合这些信息是物种划分的有力工具。
